膀胱癌患者尿液中表皮生长因子受体的表达及临床意义

Clinical significance of epidermal growth factor receptor(EGFR) m RNA levels in patients with bladder cancer

目的研究膀胱癌患者尿液中表皮生长因子受体(epidermal growth factor receptor,EGFR)m RNA的表达及临床意义。方法以60例膀胱癌患者为研究组,选择同期行健康体检的30名正常人为对照组,以荧光原位杂交(fluorescence in situ hybridization,FISH)技术测定所有受试者尿液中EGFR m RNA的表达水平,比较两组尿液中EGFR m RNA的表达并分析其表达与膀胱癌临床病理特征的关系。结果研究组尿液中EGFR m RNA的阳性表达率(71.67%)与对照组(0)比较差异有统计学意义(P<0.05)。浸润型肿瘤与浅表型肿瘤(100%vs 63.04%)、高级别肿瘤与低级别肿瘤(100%vs 62.22%)、有淋巴结转移者与无淋巴结转移者(90.91%vs 60.53%)的EGFR m RNA阳性表达率差异均有统计学意义(P均<0.05)。结论表皮生长因子受体在膀胱癌患者尿液中过表达,其过表达与肿瘤类型、病理分级及淋巴结转移密切相关,检测膀胱癌患者尿液中表皮生长因子受体的表达可作为膀胱癌诊断、肿瘤类型及肿瘤转移的评估指标。

Objective To assess the potential clinical significance of epidermal growth factor receptor （EGFR） mRNA levels in patients with bladder cancer. Methods Fluorescence in situ hybridization was used to compare EGFR mRNA levels between 60 patients with bladder cancer and 30 patients without bladder cancer. EGFR mRNA levels were also correlated with clinico- pathological features of bladder cancer. Results EGFR mRNA was detected in 71.67% of patients, compared to 0 of controls （P〈0.05）. Among patients, mRNA levels were significantly higher （P〈0.05） in those with tumor infiltration than in those with superficial cancer （100% vs 63.04%）,higher in patients in high-grade tumors than in those in low-grade tumors （100% vs 62.22%）,and higher in patients with lymph node metastasis than in those without （90.91% vs 6053%）. Conclusion Overexpression of EGFR mRNA is related to clinical stage,pathological grade and lymph node metastasis in bladder cancer, Measuring mRNA levels may help in cancer staging and in evaluating tumor metastasis.