香青兰提取物中苯丙素类和黄酮类3个化合物的含量测定

Simultaneous Determination the Contents of Phenylpropanoids and Flavonoids in Dracocephalum moldavica L.Extract

目的 建立同时测定香青兰提取物中1种苯丙素类成分（迷迭香酸）和2种黄酮类成分（木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷）的含量测定方法。方法 采用PurospherSTARLP RP-18 endcapped（4.6 mm×250 mm,5μm）色谱柱,流动相为乙睛（A）-0.5%甲酸水溶液（B）,梯度洗脱（0~28 min,20%A;28~55 min,20%→28%A;55~80 min,28%→30%A）,流速为1.0 m L·min-1,检测波长为330 nm,柱温为35℃。结果 迷迭香酸、木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷分别在2.184~21.84μg·m L-1（r=0.999 6）,3.14~31.84μg·m L-1（r=0.999 6）,7.9~39.5μg·m L-1（r=0.999 5）内呈良好线性关系。香青兰提取物迷迭香酸、木犀草素-7-O-β-D-葡萄糖醛酸苷和田蓟苷的平均回收率（n=9）分别为99.62%、99.64%和100.12%,且RSD分别为1.27%、1.05%和1.09%。结论 本方法准确、简便,重复性好。可用于同时测定香青兰提取物中木犀草素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸和田蓟苷的含量。

OBJECTIVE To establish an HPLC method for simultaneous detemination the contents of rosmarinic acid（ phenylpropanoids）,luteolin-7-O-β-D-glucuronide and tilianin（ flavonoids） in Dracocephalum moldavica L.Extract.METHODS The samples were separated on PurospherSTARLP RP-18 endcapped column（ 4.6 mm × 250 mm,5 μm） by gradient elution with acetonitrile（ A）-0.5% formic acid solution（ B）（ 0-28 min,20% A; 28-55 min,20% →28% A; 55-80 min,28% →30% A） as the mobile phase at a flow ratio of 1.0 m L·min-1.The detection wavelength was set at 330 nm and the column temperature was maintained at 35 ℃.RESULTS The calibration curves of rosmarinic acid,luteolin-7-O-β-D-glucuronide,tilianin were in good linearity over the ranges of2.184-21.84 μg·m L-1（ r = 0.999 6）,3.14-31.84 μg·m L-1（ r = 0.999 6）,7.9-39.5 μg·m L-1（ r = 0.999 5） respectively.The average recoveries were 99.62%,99.64% and 100.12% with RSD values of 1.27%,1.05% and 1.09%.CONCLUSIONThe method is reliable,simple,and accurate,and can be used for the comprehensive quality control of Dracocephalum moldavica L.extract.