亚冬眠疗法对兔急性肺损伤后炎性因子肿瘤坏死因子-α和白细胞介素-1β表达的影响

Influence of artificial sub-hibernation therapy on the expression of inflammatory cytokines tumor necrosis factor-α and interleukin-1β after acute lung injury in rabbits

目的 观察亚冬眠疗法对肺损伤后血液和肺组织灌洗液中炎性因子肿瘤坏死因子-α（TNF-α）和白细胞介素（IL）-1β表达的影响.方法 将30只8～10周龄健康雄性新西兰大白兔30只随机分为正常对照组、急性肺损伤组和急性肺损伤＋亚冬眠治疗组（亚冬眠治疗组）,建立急性肺损伤模型后,通过一般生命体征、肺损伤和炎性因子等指标评价亚冬眠治疗对急性肺损伤的影响.结果 急性肺损伤组患者伤后6h呼吸频率为（86.4±1.5）次/分、心率为（300.4±4.0）次/分,明显高于亚冬眠治疗组（P＜0.05）,而伤后6h血压为（80.8±2.1）mmHg（1 mmHg =0.133 kPa）,明显低于亚冬眠治疗组（P＜0.05）;急性肺损伤组肺组织水肿和炎性细胞浸润严重,伤后2h血清中炎性因子TNF-α和IL-1β含量分别为（652.00 ±15.53） ng/L和（259.60±4.35） ng/L,明显高于亚冬眠治疗组,且伤后6h肺组织灌洗液中炎性因子TNF-α和IL-1β含量应分别为（527.40±6.84） ng/L和（1 557.00±37.41） ng/L,明显高于亚冬眠治疗组（P＜0.05）;与急性肺损伤组比较,亚冬眠治疗可以明显减轻肺组织损伤程度,缓解外伤后各项检测指标的变化趋势（P＜0.05）,但与正常对照组比较差异有统计学意义（P＜0.05）.结论 在急性肺损伤的早期使用亚冬眠治疗可以减轻肺损伤程度,降低机体的炎性反应.

Objective To investigate the influence of hibernation therapy on inflammatory cytokines tumor necrosis factor-α （TNF-α） and interleukin （IL）-1β in the blood and lung lavage after acute lung injury.Methods Thirty male New Zealand rabbits were randomly divided into normal control group,acute lung injury （ALI） group and ALI＋ sub-hibernation therapy group.Then after the establishment of acute lung injury model,general vital signs,lung damage,inflammatory cytokines and other indicators were used to evaluate the effects of sub-hibernation therapy on curing acute lung injury.Results In ALI group,breathing rate was （86.4 ± 1.5） cpm and the heart rate was （300.4 ±4.0） cpm in 6 h after injury,which were significantly higher than the subhibernation therapy group （P ＜ 0.05）,while the blood pressure was （80.8 ±2.1） mmHg （1 mmHg =0.133 kPa） in 6 h after injury,which was significantly lower than the sub-hibernation therapy group （P ＜ 0.05）;The ALI group presented with severe pulmonary edema and inflammatory cells infiltration,the inflammatory cytokines TNF-α and IL-1β in serum were respectively （652.00 ± 15.53） ng/L and （259.60 ± 4.35） ng/L in 2 h after injury,which were significantly higher than the sub-hibernation therapy group （P ＜ 0.05）,and the inflammatory cytokines TNF-α and IL-1β in broncho-alveolar lavage fluid were respectively （527.40 ± 6.84） ng/L and （1 557.00 ±37.41） ng/L in 6 h after injury,which were significantly higher than the sub-hibernation therapy group （P ＜ 0.05）;Compared to ALI group,Sub-hibernation therapy can significantly reduce the degree of lung injury and ease the trendencies of measured parameters after acute lung injury （P ＜ 0.05）,but there are still significant differences existed compared to normal controls （P ＜ 0.05）.Conclusion In the early state after acute lung injury,sub-hibernation therapy can reduce lung injury and reduce inflammatory response after acute lung injury,which could provide experimental evidence on the choice of early treatment for acute lung injury.