微小RNA-219-5p在肝细胞肝癌中的表达及其与乙醇的关系

Expression of microRNA-219-5p in hepatocellular carcinoma and its relationship with alcohol

目的 观察微小RNA（miRNA,miR）-219-5p与乙醇、临床病理特征的关系及其在肝细胞肝癌（HCC）发生发展中的作用.方法 采用实时荧光定量聚合酶链反应（FQ-PCR）法对120例HCC患者组织中的miR-219-5p的表达进行检测,并比较其与饮酒、临床病理特征之间关系.结果 （1）肿瘤瘤体＞5 cm、组织分化差以及大量饮酒者miR-219-5p的表达显著降低（x2=10.550、3.956、6.061,P＜0.05）;（2）在Logistic回归分析中,大量饮酒史是miR-219-5p表达水平下降的主要危险因素之一[95％可信区间（CI）：1.521 ～ 17.635,P＜0.05];（3）在Cox回归模型中,miR-219-5p表达水平降低的患者具有较高的死亡风险,饮酒、甲胎蛋白（AFP）阳性、慢性肝病以及肿瘤较大者,死亡风险进一步增加（P＜0.05）.结论 miR-219-5p表达水平、血清AFP阳性、肿瘤大小、慢性肝病是HCC患者生存预后的独立危险因素,同时大量饮酒可以进一步放大上述效应.

Objective To study the roles of microRNA （miRNA,miR）-219-5p in the pathogenesis of hepatocellular carcinoma （HCC） and its relationship with alcohol and clinicopathological features.Methods The expression of miR-219-5p in 120 cases of HCC tissue samples was detected by real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）,and its relationship with alcohol and clinicopathological features was analyzed.Results （1） The expression of miR-219-5p was decreased significantly in those cancers tumors with diameter ＞ 5 cm,worse tissue differentiation and heavy drinking （x2 =10.550,3.956,and 6.061 respectively,P ＜ 0.01）;（2） The logistic regression analysis revealed thaat a history of heavy drinking was one of the major risk factors of down-regulated miR-219-5p expression level [95％ confidence interval （CI）：1.521-17.635,P ＜0.05）;（3） In the Cox regression model,the patients with the down-regulated miR-219-5p expression levels had high risk of death,and drinking,positive alpha fetal protein （AFP）,chronic liver disease and larger tumor further increased the risk for death （P ＜ 0.05）.Conclusion It is believed that the expression of miR-219-5p,serum AFP-positive,tumor size,and chronic liver disease are independent risk factors for survival of patients with HCC,while heavy drinking consumption can further amplify the effects.