结肠癌临床病理特征与Toll样受体4表达的关系

Relationship and significance of Toll-like receptor 4 expression with clinic-pathological characteristics of colon cancer

目的 检测结肠癌、溃疡性结肠炎及癌旁肠黏膜组织中Toll样受体4（TLR-4）的表达,探讨TLR-4与结肠癌发生、发展及预后的关系.方法 采用实时荧光定量聚合酶链反应（FQ-PCR）法对247例结肠癌患者癌组织、48例相对正常组织、50例溃疡性结肠炎组织中TLR-4mRNA进行检测.结果 癌组织TLR-4表达水平与淋巴结转移状态、有无远处转移、临床分期、组织学类型等临床病理特征明显相关（P＜0.05）.高表达TLR-4 mRNA患者与低表达者比较,中位总生存期（OS）明显缩短（24个月比52个月,P＜0.01）.结肠癌组织中TLR-4 mRNA的表达水平明显高于溃疡性结肠炎组织和癌旁组织（P＜0.01）.溃疡性结肠炎组织中TLR-4 mRNA表达水平明显高于癌旁组织（P＜0.01）.结论 结肠癌组织中TLR-4表达与临床分期和肿瘤转移相关.TLR-4高表达预示着结肠癌患者预后不良.

Objective To investigate the expression level Toll-like receptor 4 （TLR-4） mRNA in colon cancer tissues,ulcerative colitis and normal tissues,and to explore the significance of TLR-4 mRNA in tumorigenesis,progression and prognosis of colon cancer.Methods The mRNA of TLR-4 was detected by real-time fluorescent quantitative polymerase chain reaction （FQ-PCR） in 247 cases of cancer tissues,50 cases of ulcerative colitis tissues and 48 cases of normal tissues.The level of TLR-4 mRNA relative expression between groups were analyzed by Mann-Whitney-Wilcoxon U-Test.Survival curves were plotted using Kaplan-Meier method with Log-rank test.Results The expression level of TLR-4 mRNA in colon cancer tissues were closely related to lymph node status,presence of distant metastasis and clinical stages （P ＜ 0.05）,but not to the Karnofsky Performance Score （KPS） and histological type of the patient （P ＞ 0.05）.The median OS between patients with high levels of TLR-4 expression and low levels of TLR-4 expression had statistically significant difference （24 months vs.52 months,P ＜ 0.01）.Conclusion High expression of TLR-4 correlated with clinical stages and tumor metastasis.High expression of TLR-4 indicated a poor prognosis of colon cancer patients.