树突状细胞-细胞因子诱导的杀伤细胞免疫治疗联合化疗对转移性结直肠癌患者血清微小RNA-21和微小RNA-106a的影响

Effect of dendritic cell-cytokine-induced killer cell autologous immune cell therapy on serum microRNA-21 and microRNA-106a of metastatic colorectal cancer patients

目的 观察树突状细胞-细胞因子诱导的杀伤细胞（DC-CIK）联合化疗对转移性大肠癌患者外周血微小RNA（miRNA,miR）-21和miR-106a表达的影响.方法 将39例转移性大肠癌患者随机分为两组,DC-CIK组23例采用XELOX方案＋DC-CIK进行治疗,对照组16例采用XELOX方案进行治疗,采用实时荧光定量聚合酶链反应（FQ-PCR）方法检测治疗前及治疗3个周期后两组患者外周血miR-21和miR-106a mRNA表达的水平,并观察患者的疗效及评分.结果 经治疗3个周期后,DC-CIK组、对照组miR-21和miR-106a mRNA相对表达量均下降,差异有统计学意义（P＜0.05）;与对照组比较,DC-CIK组下降更明显（P＜0.05）.DC-CIK组有效率（73.9％）明显高于对照组（31.3％,P＜0.05）.两组Ⅲ＋Ⅳ度白细胞减少率的比较差异有统计学意义（P＜0.05）.结论 DC-CIK细胞可有效提高结直肠癌整体治疗疗效.下调miR-21和miR-106a是其重要机制之一.

Objective To explore the effect and mechanism of dendritic cell-cytokine-induced killer cell （DC-CIK） combined with chemotherapy on microRNA （miRNA,miR）-21 and miR-106a in peripheral blood of patients with metastatic colorectal cancer （CRC）.Methods Thirty-nine patients with metastic CRC were randomly divided into the treatment group （n =23,given DC-CIK combined with chemotherapy） and the control group （n =16,given chemotherapy alone）.The mRNA expression levels of miR-21 and miR-106a in peripheral blood of CRC patients were determined after 3 cycles by real-time fluorescent quantitative polymerase chain reaction （FQ-PCR）.The efficacy and Karnofsky score of each group were studied.Results The mRNA expression levels of miR-21 and miR-106a in two groups were significantly decreased （P ＜ 0.05） as conmpared with those before chemotherapy,more significantly in the treatment group than in the control group （P ＜ 0.05）.Total efficacy of treatment group （73.9％） was significantly higher than that of control group （31.3 ％,P ＜ 0.05）.There was significant difference in rate of hypolekocytosis at Ⅲ ＋ Ⅳ degree between two groups （P ＜ 0.05）.Conclusion DC-CIK can enhance the therapeutic effect probably by reducing the expression level of miR-21 mRNA.