椎板切除后硬膜粘连动物模型的建立

Establishment of a rabbit model of post-laminectomy peridural adhesion

目的 建立模仿椎板切除术后硬膜粘连、瘫痪率低、长期存活的动物模型.方法 选取新西兰兔40只,采用新的麻醉及手术方式切除第六腰椎（L6）椎体的椎板及上下椎板间孔黄韧带、硬膜外脂肪,术后观察兔一般情况,术后3个月行核磁共振（MRI）检查,术后6个月处死兔行大体观察及组织病理切片观察进行模型评价.结果 所有兔均无瘫痪,术后3个月存活率为100％,术后6个月存活率为90％;MRI示椎管后方压迫明显,正常脊髓周围间隙消失;大体观察硬膜瘢痕粘连明显,粘连等级评分均达3分;组织病理学观察见大量胶原纤维增生.结论 该方法可有效建立椎板切除后硬膜粘连动物模型,重复性好,瘫痪率低,动物可长期存活.

Objective To develop long-term survival peridural adhesion model with low paralysis rate after laminectomy in rabbits.Methods Forty New Zealand rabbits underwent laminectomy of the 6th lumber,yellow ligament were removed at the same time.Animals were observed in general after the surgery and got magnetic resonance imaging （MRI） scanning 3 months later.Rabbits were sacrificed 6 months after treatment.Peel-off testing and histological analysis were performed to assess the tenacity and the extent of adhesion formation.Results No animnal paralyzed.The survival rate was 100％ at three months after surgery and 90％ at six months.MRI showed oppression to spinal cord from dorsal tissue and the epidural space disappeared.General observation showed significantly epidural scar adhesions.Adhesion scores all reached 3 points.Histological analysis showed significantly scar tissue formation.Conclusion This kind of model can be used to study the peridural adhesion after laminectomy.