大孔吸附树脂结合半制备型高效液相色谱分离纯化栝楼果皮中的水溶性化学成分(英文)

Isolation and Purification of Water-Soluble Constituents from Trichosanthes kirilowii Maxim. Peel Using Macroporous Absorption Resin Coupled with Semi-preparative High Performance Liquid Chromatography

本文使用大孔吸附树脂结合半制备型高效液相色谱,对栝楼果皮中的水溶性化学成分进行了分离纯化。栝楼果皮水溶性粗提物经大孔吸附树脂粗分为4个部分,每一部分分别使用半制备型高效液相色谱进一步纯化,最终得到3种核苷酸及6种碱基,其化学结构经紫外光谱及核磁共振鉴定为:胞嘧啶、尿嘧啶、次黄嘌呤、鸟嘌呤、黄嘌呤、腺嘌呤、鸟苷、6-异次黄嘌呤核苷及腺苷。本文对于栝楼果皮中水溶性化学成分进行了较为系统的研究,建立起来的分离纯化方法具有简单、快速、经济和易于放大的优点,适合于天然产物中强极性活性成分的大规模制备。

In the present study,a simple and efficient preparative procedure was developed for the isolation and purification of water-soluble constituents from Trichosanthes kirilowii Maxim. peel using macroporous absorption resin followed by semi-preparative high performance liquid chromatography（ SPHPLC）. The water-soluble fraction of T. kirilowii Maxim.peel was firstly chopped up using macroporous absorption resin to yield four subfractions,and they were further purified by SPHPLC,respectively. Finally,nine compounds including three nucleosides and six nucleobases were obtained with high purities,and their structures were confirmed as cytosine,uracil,hypoxanthine,guanine,xanthine,adenine,guanosine,6-isoinosine and adenosine according to UV and NMR analysis. In this work,the chemical constituents of water-soluble fraction of T. kirilowii peel were systematically studied. The developed procedure was simple,effective,scalable and economical,it was a promising alternative procedure for large-scale preparation of polar compounds from natural products.