土壤来源链霉菌KIB-H91次级代谢产物的分离鉴定

Isolation and Identification of the Secondary Metabolites from a Soil-derived Streptomyces sp. KIB-H91

利用硅胶柱层析和制备型高效液相色谱等方法从来自土壤的一株链霉菌Streptomyces sp.KIB-H91的发酵液中分离得到4种醌类化合物,通过分析其理化性质和波谱数据,分别鉴定为endocrocin(1)、laccaic acid D(2)、sarubicin A(3)和sarubicin B(4),其中,sarubicin B(4)的13C和HMBC核磁数据为本文首次报道。抗菌敏感性试验发现化合物3对大肠杆菌ATCC 8099的最小抑制浓度(MIC)为25μg/m L,化合物4对枯草芽孢杆菌ATCC 6633的最小抑制浓度为12.5μg/m L。

Four quinone natural products,endocrocin（ 1）,laccaic acid D（ 2） and sarubicins A and B（ 3 and 4）,were isolated from the soil-derived actinomycete Streptomyces sp. KIB-H91. Their structures were elucidated on the basis of spectroscopic evidence and comparison with literature reports. Among them,the13 C NMR and HMBC data of compound4 was reported for the first time in this paper. Sarubicins A and B were the main antimicrobial components with MIC values of 25 μg / m L for 3 against Escherichia coli ATCC 8099 and of 12. 5 μg / m L for 4 against Bacillus subtilis ATCC6633.