排风藤内生真菌PFT-2发酵产物抗氧化活性研究

Antioxidant Activity of Fermentation Products of Entophytic Fungal PFT-2 in Solanum cathayanum

采用DPPH·法及Fe3+还原力法对排风藤内生真菌PFT-2提取物的抗氧化活性进行研究,首先测定发酵液乙酸乙酯萃取物和菌丝体甲醇总提物的活性,然后测定菌丝体总提物各极性部位,即石油醚、氯仿、乙酸乙酯、正丁醇,丙酮、甲醇和水层剩余物部位的活性,同时以VC作为阳性对照。结果表明:内生真菌PFT-2菌丝体甲醇总提取物各样品抗氧化活性较强,尤其是甲醇提取物部位、丙酮提取物部位和正丁醇部位;它们对DPPH·的清除作用与Vc相当,其中甲醇提取物部位IC50值为0.023 mg/m L,活性略高于VC,同时以上提取物对Fe3+也具有很强的还原能力。

DPPH· scavenging assay and ferric reducing power assay in vitro were used to evaluate the antioxidant activities of the extracts from entophytic fungal PFT-2 in Solanum cathayanum. Firstly,the antioxidant activities of ethyl acetate extract from fermentation liquid and the methanol extract of mycelium were determined. Secondly,the antioxidant activities of petroleum ether fraction,chloroform fraction,ethyl acetate fraction,n-butanol fraction,acetone fraction,methanol fraction,water surplus parts from the total methanol extract of mycelium were tested,respectively. Simultaneously VC was used as positive control. The experimental results showed that all fractions of the methanol extraction of mycelium from entophytic fungal PFT-2 in S. cathayanum had strong scavenging capacity against DPPH·,in which ethanol fraction,acetone fraction and n-butanol fraction were almost equivalent to VCfor DPPH· scavenging activity. The IC50 value of ethanol fraction for the DPPH· assay was 0. 023 mg / m L,which suggested that antioxidant activity of ethanol fraction was even stronger than that of VC. Meanwhile,the above fractions all revealed strong Fe3 ＋reducing power.