栝楼果皮中腺嘌呤、鸟苷、瓜氨酸动态积累规律研究

Dynamic Accumulation of Adenine,Guanine nucleoside and Citrulline in Trichosanthes kirilowii Peel

对栝楼果皮生长期间主要活性成分腺嘌呤、鸟苷、瓜氨酸含量进行分析,探索活性成分积累规律。以长清产栝楼果皮为研究对象,从2013年7月3日到2013年10月26日采用高效液相色谱法进行腺嘌呤、鸟苷、瓜氨酸含量测定。实验表明腺嘌呤、鸟苷、瓜氨酸在生长期含量变化趋势是先降后升再降,至果皮表面颜色稍微泛黄时,腺嘌呤含量达到最高值0.1893 mg/g,至果皮表面颜色基本全黄、仅维管束凸起处为绿色时鸟苷含量达到最高值0.4128 mg/g,瓜氨酸含量达到最高值12.8834 mg/g。提示不同生长期栝楼果皮中腺嘌呤、鸟苷、瓜氨酸含量变化较大,果皮表面颜色基本为黄色仅维管束凸起处为绿色时为山东长清瓜蒌皮药材最佳采收期。

To explore the general law of dynamic accumulation of the active components in the peel of Trichosanthes kirilowii Maxim.,the contents of adenine,guanine nucleoside and citrulline were investigated during its different growth periods. T. kirilowii peel in Changqing district from the periods of July 3rd 2013 to October 26 th 2013 was used as the subjects of analysis. The results indicated that the contents of adenine,guanine nucleoside and citrulline declined at beginning then rose,then declined during the growth periods. When the surface color was slightly yellow,the content of adenine reached the highest 0. 1893 mg / g; When the surface color basic all yellow,only vascular bundle was green,the content of guanine nucleoside reached the highest 0. 4128 mg / g,the content of citrulline reached the highest 12. 8834 mg / g.In conclusion,the contents of adenine,guanine nucleoside,citrulline in T. kirilowii peel from different growth periods differed significantly. The peel color yellow only vascular bundle green was suggested to be the best picking time for T. kirilowii peel.