藏药五脉绿绒蒿不同部位红外光谱的识别

Analysis of Different Parts of Tibetan Medicine Meconopsis quintuplinervia Regel by FT-IR

采用傅立叶变换红外光谱法对藏药五脉绿绒蒿花、花梗、叶以及全草进行了红外光谱图的识别分析。对五脉绿绒蒿在4000~400 cm-1范围内进行了红外光谱扫描,并对主要吸收谱带进行了基团的归属分析。五脉绿绒蒿红外光谱特征的分析结果表明,不同部位的一维光谱和二阶导数谱有明显差异。一维红外光谱谱图中主要特征谱带的相对强度比值,可对不同部位进行区分。二阶导数谱图的1517~1471 cm-1和1162~1107 cm-1波段是区分其不同部位的主要特征波段。因此,红外光谱能够快速、无损地对五脉绿绒蒿不同部位进行鉴别,为藏药五脉绿绒蒿不同部位的成分差异分析提供了一种科学有效的方法。

In this study,different parts of Meconopsis quintuplinervia were discriminated and identified by Fourier transform infrared spectroscopy（ FT-IR） coupled with second derivative infrared spectroscopy. The findings indicated that different parts of M. quintuplinervia samples had dissimilar fingerprint characters in FT-IR spectra. Such spectral technique can provide structural information of the complicated samples. There were differences in the ratio of relative intensity of the characteristic peaks of FT-IR. The difference was amplified by the second derivative spectrum for the peak shape in 1517-1471 cm-1and 1162-1107 cm-1. In conclusion,FT-IR spectroscopy combined with second derivative spectroscopy provided a powerful and useful approach for the identification of traditional medicines.