油樟油对油樟内生真菌中活性化合物影响

Effects of Cinnamomum longepaniculatum Oil on the Active Components of Endophytic Fungi

YY26、YG42和YG71是从油樟中分离得到的三株内生真菌。本研究采用GC-FID、GC-MS技术,以油樟油作为诱导物,研究YY26、YG42、YG71菌株在不同培养基条件下对菌株中活性化合物1,8-桉叶油素、松油烯-4-醇、a-松油醇、r-松油烯产量的影响。结果表明:在PDB培养基中,一方面,油樟油可促进YY26、YG42菌株积累1,8-桉叶油素等物质,其中对1,8-桉叶油素的促进最强,分别提高1.41倍和1.30倍;另一方面,油樟油抑制YG71菌株中以上4种物质的积累。在察氏培养基中,油樟油对YY26、YG42及YG71菌株中4种物质的积累没有明显的促进作用。该研究为植物有用代谢产物的规模化生产提供了理论依据,有较强的实践意义。

YY26,YG42 and YG71 were three endophytic fungi strains isolated from Cinnamomum longepaniculatum.With C. longepaniculatum oil as inductor,GC-FID and GC-MS were used to analyze the accumulation of 1,8-cineole,terpinen-4-ol,a-terpineol and r-Terpinene in YY26,YG42,and YG71 strains under different media conditions. The experimental results indicated that in PDB medium C. longepaniculatum oil can promote accumulation of 1,8-cineole in YY26,YG42 strains and the content of 1,8-cineole increased by 1. 41 and 1. 30 times respectively,while 1,8-cineole were repressed in strains YG71 in PDB medium. C. longepaniculatum oil had no obvious promotion effect on the 4 compounds in czapek＇s medium. This study provided scientific basis for the large-scale production of natural metabolites.