摘要
目的探讨T淋巴细胞表面共刺激分子在慢性乙型肝炎(CHB)患者外周血中的表达及其临床意义。方法选取我院在2013年3月至2014年3月期间收治的126例CHB患者和90例健康对照者作为研究对象,利用流式细胞术和定量PCR检测CHB患者和健康对照者外周血中T淋巴细胞表面共刺激分子CD28、细胞毒性T淋巴细胞抗原(CTLA)-4、程序性死亡分子(PD)-1、T细胞免疫球蛋白粘蛋白分子(Tim)-3及T淋巴细胞亚群的表达变化,并进行比较分析。结果CHB患者外周血CD3^+、CD4^+T细胞百分率及CD4^+/CD8^+比值均低于对照组,而CD8^+T细胞百分率高于对照组,组间比较差异具有统计学意义(分别t=4.0868,7.4660,5.5207和3.8160,均P〈0.01);同时,HBVDNV阳性组患者外周血CD3^+、CD4^+T细胞百分率及CD4^+/CD8^+比值均低于HBVDNA阴性组,而CD8^+T细胞百分率高于HBVDNA阴性组,组问比较差异具有统计学意义(分别t=5.1567,2.7884,2.9987和2.9087,均P〈0.01);此外,CHB患者外周血共刺激分子CD28^+及CTLA-4^+的表达量均低于正常对照组,而PD-1和Tim-3的表达量高于对照组,差异具有统计学意义(分别t=6.0546,5.0669和6.3006,8.2151,均P〈0.01);同时,HBVDNA阳性高拷贝组患者外周血共刺激分子CD28及cTLA-4的表达量均低于低拷贝组,而PD-1和Tim-3的表达量高于低拷贝组,组间比较差异具有统计学意义(分别t=3.1293,2.7459,2.8661,2.7960,均P〈0.01)。结论外周血T淋巴细胞亚群及其表面共刺激分子的表达水平对于指导CHB患者的临床治疗和判断预后具有一定的临床意义。
Objective To investigate the changes in the expression of co-stimulatory molecules in the peripheral blood T lymphocyte subsets in patients with chronic hepatitis B infection. Methods 126 patients with chronic hepatitis B who got medical treatment in our hospital from 2013 March to 2014 March, together with 90 healthy subjects were enrolled in our case-control study,We determined the expression levels of CD28, cytotoxic T lymphocyte antigen(CTLA)-4, programmed cell death (PD)-1, T cell immunoglobulin domain and mucin domaim(Tim)-3, CD3^+, CD4^+ , CD8^+, CD4^+/CD8^+ on peripheral blood T lymphocyte subsets with flow cy- tometry and Real-time PCR. Results The percentage of CD3^+ and CD4^+ T cells, CD4^+/CD8^+ ratio in patients with chronic hepatitis B were significantly lower than those in healthy control, and the percentage of CD8^+ T cells was significantly higher than in healthy conntrol (t = 4. 0868,7. 4660,5. 5207 and 3. 8160, all P 〈 0. 01 ). Meanwhile,compared with the HBV DNA-negative patients with chronic hepatitis B infection( CHB), the HBV DNA-positive patients with CHB had a significant change in the percentage of CD3^+, CD^+ and CD8^+ T cells , as well as in the CD4^+/CD8^+ ratio ( t = 5. 1567,2. 7884,2.9987and 2. 9087, all P 〈 0.01 ). Besides, the percentage of CD28 and CTLA-4 in patients with chronic hepatitis B were significantly lower than those in healthy control, and the percentage of PD-I and Tim-3 were significantly higher than those in healthy conntrol( t = 6.0546,5. 0669 and 6.3006,8.2151, all P 〈 0.01 ). The percentage of CD28 and CTLA-4 in patients with high copies group were significantly lower than those in low copies group,and the percentage of PD-1 and Tim-3 were significantly higher than those in low copy group (t = 3. 1293,2.7459,2.8661,2. 7960, all P 〈 0. 01 ). Conclusion The changes in the expression of co-stimulatory molecules on the peripheral blood T lymphocyte subsets may guide the clinical therapy and judge the prognosis in patients with chronic hepatitis B infection.
出处
《国际免疫学杂志》
CAS
2015年第4期325-328,332,共5页
International Journal of Immunology
关键词
慢性乙型肝炎
外周血T淋巴细胞
共刺激分子
流式细胞术
Chronic hepatitis B
T lymphocyte cell subsets in peripheral blood
Co-stimulatory moleculesl
flow cytometry