硫化氢对肥胖小鼠肝脂质蓄积的影响

Effect of hydrogen sulfide on hepatic lipid accumulation in obese mice

目的探讨硫化氢对肥胖小鼠肝脏脂质蓄积的影响。方法 C57BL/6J小鼠,随机分为对照组、模型组、硫化氢干预组。对照组喂普通饲料,模型组和硫化氢干预组喂高脂饲料。从第13周开始,硫化氢干预组注射硫氢化钠,剂量为50μmol·kg-1·d-1,模型组注射等量生理盐水,16周末处死动物。肝脏组织匀浆,取上清,做生化检测,测量各组小鼠肝组织中甘油三酯、胆固醇含量;肝脏石蜡切片做H&E染色观察肝组织一般形态;冷冻切片,油红染色观察肝组织脂质蓄积情况;肝脏新鲜冰冻组织提取RNA,PCR检测小鼠肝脏中CPT-1、FAS的基因表达情况,并用ELISA法检测CPT-1、FAS的活性。结果模型组、硫化氢干预组小鼠体重明显高于对照组。与模型组相比,硫化氢干预组小鼠体重减轻;肝组织内甘油三酯、胆固醇含量明显下降;肝组织病理变化程度减轻,脂质蓄积减少;肝脏CPT-1表达及活性增高,FAS表达及活性降低。结论硫化氢可以降低肥胖小鼠肝组织脂肪含量,减轻肝脂肪变性程度,其机制可能与肝组织CPT-1表达增加、FAS表达下降有关。

Aim To investigate the effect of hydrogen sulfide on hepatic lipid accumulation in obese mice. Methods C57 BL/6 J mice were randomly divided into control group, model group, and NaHS group. The mice of the control group were fed with normal diet. The mice of the model group and the NaHS group were fed with high-fat diet. From the thirteenth week, the mice of NaHS group were injected intraperitoneally with NaHS (H2S donor) in a dose of 50 μmol·kg-1 per day for 4 weeks and the mice of the model group were injected with the same volume of saline. All mice were sacrificed at the end of the 16th week. The tis-sues of liver were homogenized and centrifugated. The supernatants were used for the determination of triglyc-eride and cholesterol in liver. The morphology of liver was tested by H&E staining. Liver lipid accumulation was determined by oil red staining. Total RNA was ex-tracted from frozen tissue of liver. PCR was used to de-tect CPT-1 , FAS gene expression and ELISA method was used to detect CPT-1,FAS activity in mice liver. Results The body weight of the mice from NaHS group and model group was bigger than that of the mice from control group. Compared with the model group, the body weight of the mice from NaHS group was less;the content of triglyceride and cholesterol in liver was lower; the degree of liver tissue pathological changes and lipid accumulation were alleviated; CPT-1 expres-sion and activity were increased; FAS expression and activity were decreased. Conclusions These data in-dicate that hydrogen sulfide can reduce the lipid con-tent of liver tissue in obese mice and alleviate fatty liv-er. The mechanism may be associated with the in-creased expression of CPT-1 and the decreased expres-sion of FAS in liver.