牡荆叶中1个新黄酮苷类化合物

A new flavonoid glycoside from leaves of Vitex negundo var.cannabifolia

目的 研究牡荆Vitex negundo var.cannabifolia叶中黄酮苷类化学成分。方法 采用硅胶、Sephadex LH-20和ODS等柱色谱技术,对牡荆叶的化学成分进行分离、纯化,通过其理化性质及MS、NMR等谱学数据鉴定化合物的结构。结果 从牡荆叶95%乙醇提取物的醋酸乙酯萃取部位分离得到7个黄酮苷类化合物,分别鉴定为木犀草素-4′-O-（6″-O-对羟基苯甲酰基）-β-D-葡萄糖苷（1）、木犀草素-7-O-（6″-O-对羟基苯甲酰基）-β-D-葡萄糖苷（2）、木犀草素-6-C-（6”-O-反式-咖啡酰基）-β-D-葡萄糖苷（3）、木犀草素-6-C-（2″-O-反式-咖啡酰基）-β-D-葡萄糖苷（4）、perfoliatuminA（5）、异牡荆素（6）、木犀草素-7-O-β-D-葡萄糖苷（7）。结论 化合物1为新化合物,命名为牡荆宁G;化合物2~4、7为首次从该植物中分离得到,化合物5为首次从牡荆属植物中分离得到。

Objective To investigate the flavonoid glycosides from the leaves of Vitex negundo var.cannabifolia.Methods Column chromatography including silica gel,Sephadex LH-20,and ODS was used to separate and purify the chemical constituents,and their structures were elucidated by physicochemical properties,MS,and NMR spectroscopic data.Results Seven flavonoid glycosides were obtained from the ethyl acetate layer of 95%EtOH extract of the leaves of V.negundo var.cannabifolia,and identified as luteolin-4＇-O-（6 ＂-O-p-hydroxybenzoyl）-β-D-glucoside（1）,luteolin-7-O-（6＂-O-p-hydroxybenzoyl）-β-D-glucoside（2）,luteolin-6-C-（6 ＂-O-trans-caffeoyl）-β-D-glucoside（3）,luteolin-6-C-（2＂-O-trans-caffeoyl）-β-D-glucoside（4）,perfoliatumin A（5）,isovitexin（6）,and luteolin-7-O-β-D-glucoside（7）.Conclusion Compound 1 is a new compound named cannabifolin G;Compounds 2—4 and 7 are obtained from this plant for the first time;Compound 5 is firstly isolated from the plants in Vitex L.