香花藤化学成分的研究

Chemical constituents of Aganosma marginata

目的对香花藤Aganosma marginata全株化学成分进行研究。方法采用硅胶色谱柱、Sephadex LH20色谱柱、MCI色谱柱等方法进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构。结果从香花藤70%乙醇提取物中分离得到23个化合物,分别鉴定为periseoside C(1)、3-O-β-D-吡喃葡萄糖-3β,15α-二羟基孕甾-5-烯-20-酮(2)、3β,20α-二羟基-5-烯-孕甾(3)、24-methylstigmast-5-en-3-ol(4)、29-norcycloart-23-ene-3,25-diol(5)、29-norcycloartan-3-ol(6)、京尼平苷酸(7)、丁香树脂醇(8)、丁香脂素-4,4′-O-双-β-D-吡喃葡萄糖苷(9)、丁香酸-4-O-β-D-葡萄糖苷(10)、水杨酸(11)、东莨菪内酯(12)、壬二酸(13)、3-O-[β-D-xylopyranosyl]-(1→4)-β-D-allopyranoside-14-hydroxycard-20(22)-enolide(14)、bis(2-ethylhexyl)phthalate(15)、山柰酚3-O-α-L-吡喃鼠李糖-(1→4)-α-L-吡喃葡萄糖苷(16)、山柰酚3-O-α-L-吡喃葡萄糖-(1→2)-β-D-吡喃葡萄糖苷(17)、山柰酚3-O-α-L-吡喃鼠李糖-(1→4)-[α-L-吡喃鼠李糖-(1→4)]-β-D-吡喃葡萄糖苷(18)、山柰酚3-O-α-L-吡喃鼠李糖-(1→4)-[β-D-吡喃葡萄糖-(1→4)]-β-D-吡喃葡萄糖苷(19)、二十六碳酸-1-甘油酯(20)、5,8,12-trihydroxy-9-octadecenoic acid(21)、(2S,3S,4R)-phytosphingosine(22)、水粉覃素(23)。结论所有化合物均为首次从该属植物中分离得到。

Objective To investigate the chemical constituents from Aganosma marginata and to provide the material basis for the quality control.Methods The chemical constituents were separated and purified by silica gel Sephadex LH-20,MCI.Their structures were determined by physicochemical properties and spectral data analyses.Results Twenty three compounds were isolated from A.marginata and identified as：periseoside C（1）,3-O-β-D-glucopyranosyl-3β,15α-dihydroxypregn-5-en-20-one（2）,3β,20α-dihydroxy-5-en-pregane（3）,28-methylstigmast-5-en-3-ol（4）,29-norcycloart-23-ene-3,25-diol（5）,29-norcycloartan-3-ol（6）,geniposidic acid（7）,syringaresinol（8）,syringaresinol-4,4＇-O-bis-β-D-glucopyranoside（9）,syringic acid 4-O-β-D-glucopyranoside（10）,salicylic acid（11）,scopoletin（12）,azelaic acid（13）,3-O-[β-D-xylopyranosyl]-（l→4）-β-D-allopyranoside-14-hydroxycard-20（22）-enolide（14）,bis（2-ethylhexyl） phthalate（15）,kaempferol-3-O-α-L-rharnnopyranosyl-（1→4）-α-L-glucopyranoside（16）,kaempferol-3-O-α-Lglucopyranoside-（1→2）-β-D-glucopyranoside（17）,kaempferol-3-O-α-L-rhamnopyranosyl-（1→4）-[α-L-rhamnopyranosyl（1→4）]-β-D-glucopyranoside（18）,kaempferol-3-O-α-L-rhamnopyranosyl-（1→4）-[β-D-glucopyranosyl-（1→4）]-β-D-glucopyranoside（19）,hexacosanoicacid 1-carbonate（20）,5,8,12-trihydroxy-9-octadecenoic acid（21）,（2S,3S,4R）-phytosphingosine（22）,and nebularine（23）.Conclusion All the compounds are obtained from plants of Aganosma G.Don for the first time.