摘要
[目的]从转录水平揭示ALMT和MATE基因在大豆耐铝中的作用,为遗传改良获得抗性种质提供依据。[方法]对2个大豆品种进行Al处理,取胁迫后6 h(短期)、2 d(中期)和12 d(长期)的根尖提取RNA,采用qRT-PCR检测耐铝相关基因的时空表达。[结果]以MTP和UBC2为内参时,ALMT基因在铝处理2 d后的BX10中表达量与对照相比变化最大,在BD2中处理12 d后的表达量最大;当使用不同的内参校准时,MATE在同一个品种中的表达量存在差异,且在同一品种处理的3个时间点的变化趋势也不一致。[结论]不同的内参基因进行校准时,基因的表达量不同,使用2个或2个以上的内参基因进行校正能够提高结果的准确性,基因的表达变化为研究植物耐铝机制提供理论基础。
[Objective] Reveal the effect of ALMT and MATE gene of soybean against to aluminum on transcription level,provide evidence for genetic improvement and acquired resistance germplasm. [Method] Extracted RNA from root tips after 6 h（ short-term）,2 d（ medium）,12d（ long-term） Al treatment on two soybean varieties,detect the the expression of genes using qRT-PCR. [Result] When using MTP and UBC2 as reference gene,ALMT has higher change in BX10 after 2d＇s treatment with Al toxicity than 6h＇s and 12d＇s. The expression of ALMT after 12d＇s treatment is higher than 6h＇s and 2d＇s. The expression of MATE has higher changes than control in three times,the highest change is 2. 50 compared to control,and the change trends was distinct in three points. MATE not only has different expression trends,but also has different expression in BD2. [Conclusion]The expression was different when using different reference gene to normalized. Using two or more reference gene can increase the accuracy of result. The changes in gene expression can provide a theoretical basis for research the plant mechanisms to aluminum tolerance.
出处
《安徽农业科学》
CAS
2015年第19期34-36,共3页
Journal of Anhui Agricultural Sciences
基金
广西研究生教育创新计划项目(YCSZ2014031)
关键词
大豆
铝毒
基因表达
Soybean
Al toxicity
Gene expression
