饲用枯草芽孢杆菌发酵工艺的优化及菌剂制备

Fermentation Process' s Optimization of Forage Bacillus subtilis and the Preparation of Bacterium Agent

[目的]为饲用枯草芽孢杆菌的大规模生产奠定基础。[方法]通过单因素试验和正交试验,对发酵培养基配方及其发酵条件进行优化。[结果]饲用枯草芽孢杆菌的最佳培养基为：葡萄糖3%、酵母浸膏1.5%、磷酸氢二钠0.2%、磷酸二氢钠0.1%、硫酸镁0.1%、酵母浸粉0.5%。饲用枯草芽孢杆菌的的最适发酵条件为：温度38℃,初始p H 7.0~7.2,摇床转速180 r/min,接种量7%。选择木屑质量与发酵液体积（m/V）的混合比为3∶5,继续高温发酵16 h,芽饱产量可达到5.18×1010CFU/g。[结论]该研究可为饲用枯草芽孢杆菌的工业化生产提供了数据支持。

[Objective]The research aimed to lay the foundation for the large-scale production of forage Bacillus subtilis. [Method]Using the single factor and orthogonal test,the formula and the fermentation conditions of culture medium were optimized. [Result] The optimum medium for forage B. subtilis was as follows： 3% glucose,1. 5% yeast extract,0. 2% sodium dimetallic phosphate,0. 1% disodium hydrogen phosphate,0. 1% magnesium sulfate,0. 5% yeast powder. The optimal fermentation conditions were as follows： temperature of 38 ℃,initial p H of 7. 0- 7. 2,rotation speed of 180 r / min,inoculation amount of 7%. The mixing ratio of wood quality and the volume of fermentation broth was 3∶5,the high-temperature fermentation continued for 16 h,the yield of bud can reach 5. 18 × 1010 CFU / g. [Conclusion] The research could provide data support for the industrial production of forage B. subtilis.