摘要
研究建立了一种高效液相色谱法同时测定膨化食品中的柠檬黄、苋菜红、Et落黄、诱惑红、胭脂红和亮蓝6种合成色素的方法。膨化食品经石油醚脱脂，α-淀粉酶水解，聚酰胺粉净化。采用Waters Symmetry Shield C18色谱柱（4．6mm×250mm，5gm），以甲醇和0．02mol／L乙酸铵为流动相，梯度洗脱，用二极管阵列检测器记录各个色谱峰的紫外吸收光谱，色谱检测波长为254nm。结果表明，各色素1．0μg／m：～50gg／mL线性关系良好，相关系数为0．9998～0．9999;加标回收率在90．5％～101．2％之间。本方法操作简便，准确可靠，适用于膨化食品中合成色素的测定。
To establish a method for simultaneous determination of five synthesized pigments （lemon yellow, amaranth, sunset yellow, allura red , carmine and bright blue） in puffed food by high-performance liquid chromatography. Puffed food were defatted by petroleum ether, hydrolyzed by a-amylase and purified by polyamide. The separation was performed on a Waters Symmetry Shield C18 （4.6mm ×250mm, 5μm） column and gradient eluted with methanol-0.02 mol/L ammonium acetate solution .The detection wavelength was 254 nm and the peaks of UV spectra were captured by photo-diode array detector. Results showed that the method displayed good linearity with correlation coefficient between 0.9998 - 0.9999.The average recoveries of spiked sample ranged from 90.5% to 101.2%.This method is simple, accurate and reliable and can be used for determination of the synthesized pigments in Puffed food.
出处
《中国食品添加剂》
CAS
北大核心
2015年第6期184-187,共4页
China Food Additives
