一个显性卷叶突变体z2的遗传分析与精细定位

Genetic Analysis and Gene Mapping of a Dominant Rolled Leaf Mutant z2 in Rice

叶片的适度卷曲对水稻理想株型育种具有重要意义。在水稻T-DNA插入突变体库中获得一个叶片外卷突变体z2,突变体表型出现在分蘖期;与野生型相比,农艺性状无明显差异,光合作用效率高于野生型。对突变体和野生型的石蜡切片研究表明,突变体叶片泡状细胞数量(8-9个)明显多于野生型(4-5个)。z2卷叶性状遗传稳定,由一对显性核基因控制。以z2纯合突变体为母本,籼稻Dular为父本进行杂交构建F2代定位群体,利用图位克隆的方法,将该基因初定位于水稻第2号染色体的In Del1812与In Del1870标记之间,物理距离为580 kb。

Moderate rolled leaf is significant in ideotype breeding of rice. An outward rolled leaf mutant z2 was discovered from the rice T-DNA insertion mutant library, and its leaves became curly during tillering stage. Compared with the wild type, there was no significant difference in its main agronomic traits, and its photosynthetic was more efficient. Paraffin sections showed that the number of bulliform cells （8-9）in the mutant was higher than that in the wild type（4-5）. Through successive selfing, phenotype of z2 was stable and was controlled by a pair of dominant nuclear genes. We made the combination of z2/Dular to establish F2 mapping population, and used map-based cloning method to locate the gene on rice chromosome 2 between maker InDel1812 and InDel1870 with 580 kb physical distance.