北京鸭胚胎后肾间充质干细胞分离培养及鉴定

Isolation,Culture and Identification of Beijing Duck Embryonic Metanephric Mesenchymal Stem Cells

建立禽类后肾间充质干细胞(Metanephric mesenchymal stem cells,MMSCs)体外培养体系,研究其生物学特性和多向分化潜能。采用酶消化法分离北京鸭胚胎MMSCs,绘制生长曲线,通过免疫荧光和RT-PCR对MMSCs进行鉴定,诱导MMSCs向脂肪细胞和胰岛细胞分化。结果表明,鸭胚胎MMSCs具有良好的增殖活力,表达间充质干细胞(Mesenchymal stem cells,MSCs)特异性标志物,并诱导分化为脂肪细胞和胰岛细胞。北京鸭胚胎MMSCs在体外具有较强的自我更新能力及多向分化潜能,可作为组织工程的种子细胞进行保存。

This study aimed to establishin vitro culture system for the poultry metanephric mesenchymal stem cells（MMSCs）, and study their biological characteristics and multi-directional differentiation potential. Enzyme digestion method was applied to separate Beijing duck embryo MMSCs and to draw the growth curve. MMSCs were identified by immunofluorescence and RT-PCR. MMSCs were induced to differentiate into adipocytes and islet cells. The results showed that MMSCs had solid proliferative activity and could express MSCs specific markers, and be induced to differentiate into adipocytes and islet cells. In summary, Beijing duck embryo MMSCs have a strong self-renewing ability and multi-directional differentiation potentialin vitro, and can be saved as seed cells for tissue engineering.