市售橙汁抗氧化活性物质的研究

Identification of antioxidant compounds in commercial orange juice

利用1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl radical,DPPH)法和铁离子还原能力(Ferric reducing antioxidant power,FRAP)法、福林酚法以及超高效液相-四极杆飞行时间串联质谱(Ultra-high performance liquid chromatography-quadruple time-of-flight-mass spectrometry,UPLC-QTOF-MS)法,分别对市售橙汁的抗氧化活性、总酚含量以及抗氧化活性物质种类进行了研究,以达到明确橙汁的抗氧化活性有无,以及研究其主要抗氧化活性物质种类的目的。实验结果表明橙汁甲醇提取液具有清除DPPH自由基的能力[(1023±9.56)μg/m L,VCEAC(Vitamin cequivalent antioxidant capaciy)]和还原铁离子能力[(804.49±7.57)μg/m L,VCEAC],并且总酚含量丰富[(601.57±9.92)μg/m L,阿魏酸当量]。橙汁中鉴定出的主要的酚类化合物为柚皮素和橙皮苷,其中柚皮素是主要的抗氧化活性物质之一。

DPPH（1,1-Diphenyl-2-picrylhydrazyl radical） method,FRAP（Ferric reducing antioxidant power） method, Folin-Ciocalteu assay and Ultra-high performance liquid chromatography-quadruple time-of-flight mass spectrometry（UPLC-QTOF-MS） were applied in this paper to analyze the antioxidant capacity of commercial orange juice and the antioxidants inside. The results showed that the commercial orange juice could scavenge the DPPH free radical [（1023±9.56）μg/mL,VCEAC（Vitamin cequivalent antioxidant capaciy）],and reduce iron ion [（804.49±7.57）μg/mL,VCEAC]. The phenolic content was rich [（601.57±9.92）lJg/mL,Ferulic acid equivalent]. The compounds identified included narigenin and hesperidin. And the narigenin was one of the main antioxidants.