响应面法优化艾叶精油的提取工艺及其化学成分分析

Study on optimization of microwave-assisted extraction and chemical composition analysis of essential oil from Artemisia argyi leaves

采用微波辅助水蒸气蒸馏法提取艾叶精油,在单因素实验基础上,通过响应面法优化艾叶精油提取的工艺条件,气相色谱-质谱法对精油的化学成分进行分析,并应用峰面积归一化法测定成分的相对百分含量。结果表明:最佳工艺条件为:微波处理时间94s,微波功率480W,蒸馏时间2.2h,Na Cl浓度5%,液料比5∶1,提取含量为2.58mg/g。主要成分为4-萜烯醇(18.75%)、6-芹子烯-4-醇(11.74%)、桉油醇(9.10%)、石竹烯氧化物(8.01%)、香芹醇(6.27%)、3,3,6-三甲基-1,4-庚二烯-6-醇(5.21%)。验证实验表明所选的工艺条件合理可行。

The microwave-assisted steam distillation extraction of essential oil from Artemisia argyi leaves was optimized for achieving maximum oil yield by response surface methodology based on single factor experiment. And chemical composition of the extracted oil was analyzed by gas chromatography-mass spectrometry（GC- MS）. Their total content was determined by the area normalization. The optimal conditions for oil extraction were found as follows：microwave power was 480W,irradiation time was 94s liquid-material ratio 5：1,distillation duration 2.2h,and concentration of NaCl was 5%. The resultant oil yield was up to 2.58mg/g. The main compouds were 3-Cyclohexen- 1 -ol, 4-methyl- 1 - （ 1 -methylethyl ） - （ 18.75% ）, Selina-6-en-4-ol （ 11.74% ）, Eucalyptol （9.10%）, Caryophyllene oxide （ 8.01% ）, 2-Cyclohexen- 1 -el, 2-methyl-5- （ 1 - methylethenyl ） -cis- （6.27%）, 3,3,6-Trimethyl- 1,4-heptadien-6-ol （5.21%）. It turned out that the selected process conditions was feasible by the verification test.