摘要
以裸燕麦籽粒为原料,利用水提取燕麦蛋白中的抗真菌蛋白(antifungal protein),用打孔法观察并测量抑菌圈直径,在单因素实验的基础上,应用响应面分析法,探讨提取时间、提取温度、p H以及液料比对燕麦抗真菌蛋白抑菌性的影响,确定燕麦抗真菌蛋白的最佳水提工艺。实验结果:当提取时间12h,提取温度30℃,p H7.5,液料比12∶1m L/g时,抑菌圈直径达到最大为18.10mm。
Antifungal proteins were extracted from seeds of naked oat by water extraction method. The antifungal activity of the protein was evaluated by hole method. Based on single factor method, response surface analysis were used to explore the effects of extraction time,extraction temperature,pH and liquid-to-solid ratio on antifungal activity of oat antifungal protein. These methods determined the optimal water extraction conditions of oat antifungal protein. Results.The optimal extraction time,extraction temperature,pH and liquid-to-solid ratio were 12h,30%,7.5,12：1mL/g. The actual maximum diameter of inhibition zone was 18.10mm.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第14期312-316,共5页
Science and Technology of Food Industry
关键词
燕麦
抗真菌蛋白
水提取法
响应面分析法
oat
antifungal protein
water extraction method
response surface anaylsis
