青钱柳抑制α-葡萄糖苷酶有效成分筛选及其对Ⅱ型糖尿病小鼠血糖的影响

Screening the active fraction from Cyclocaryapaliurus(Batal.) Ijinskaja which inhibits α-glucosidase and study its effects on hyperglycemia in mice with type Ⅱdiabetes mellitus

研究青钱柳(Cyclocaryapaliurus(Batal.)Ijinskaja)抑制α-葡萄糖苷酶有效成分及其对正常小鼠糖耐量的改善和对Ⅱ型糖尿病小鼠的空腹血糖(FBG)、血清总胆固醇(TC)和总甘油三酯(TG)的影响。用聚酰胺柱富集青钱柳醇提取物中总黄酮和总三萜,用水提醇沉法和sevag法制备青钱柳总多糖;以阿卡波糖为对照体外检测各组分对α-葡萄糖苷酶的抑制作用;通过STZ联合高糖高脂饲料诱导Ⅱ型糖尿病小鼠模型,分不同剂量组给青钱柳总黄酮(CPF),四周后检测各生化指标。结果表明:富集后CPF中黄酮含量达到55.66%,对α-葡萄糖苷酶有很好的抑制作用,其IC50值60.477mg/L接近阿卡波糖的IC50值58.608mg/L;能明显改善正常小鼠糖耐量,降低餐后血糖;能降低糖尿病小鼠的FBG、TC、TG。CPF能抑制α-葡萄糖苷酶活性,降低糖尿病小鼠的血糖。

The active fraction of Cyclocaryapafiurus（Batal.） Ijinskaja which inhibited α-glucosidase were screened, and studied its effects on hyperglycemiainmice with type II diabetes mellitus. Column chromatography techniques were applied to isolate the total flavonoids and the total triterpenes. The preparation of total polysaccharides employed water extracting-alcohol precipitating and sevag method.The activity of inhibiting α-glucosidase was evaluated compared with acarbose in vitro. The oral glucose tolerance test（OGTT） in normal mice was applied to estimate the affection on postprandial blood sugar of total flavonoids from Cyclocaryapafiurus（CPF）. The model of type II diabetes mellitus was established by treating the mice with high-fat diet（HFD） and streptozotocin （STZ）. CPF was administered orally at different doses to the diabetic mice. Fasting blood glucose （FBG）,total glycerin （TG）,total cholesterol （TC） were evaluated in normal and diabetic mice after four weeks. The concentration of total flavonoids from Cyclocaryapaliurus was 55.66% ,it significantly inhibited glycosidic enzyme activityand its IC50 value of 60.477mg/L approached acarbose＇s ICso value of 58.608mg/L. CPF markedly improved OGTT of normal mice and reduced FBG,TC and TG in mice with type Ⅱ diabetes mellitus. Hypoglycemic effect of Cyclocaryapafiurus associated with glycosidic enzyme inhibitory activity of flavonoids.