水稻矮秆多分蘖突变体bf370的遗传分析和基因定位

Genetic Analysis and Mapping of a Dwarf and High-tillering Mutant bf370 in Rice

通过中子辐射诱变早籼稻品种红矮B,获得矮秆多分蘖突变体bf370。该突变体与野生型相比表现为植株矮化,分蘖极多。bf370在全生育期内的分蘖数达200个左右,是野生型分蘖数量的14倍以上。遗传分析表明该矮秆多分蘖突变体表型受一对隐性核基因控制。利用突变体bf370与日本晴杂交构建的F2群体将突变基因定位到第1染色体长臂Indel 4与Indel 10之间398kb区域内。测序分析发现,与野生型相比突变体该区段内的D10基因在第2外显子上缺失66bp碱基,导致D10蛋白RPE65结构域22个氨基酸缺失。结合D10其他突变体表型推断,bf370表型极有可能由D10突变所致。

A dwarf and high-tillering mutant bf370 was obtained from the indica rice variety Hong＇ai B by neutron radiation.Compared with the wild type,the mutant bf370 was characterized by dwarf plant and more tillers.The number of tillers in bf370 was about 200,which was 13 times more than that in the wild type.Genetic analysis indicated that this phenotype was controlled by a single recessive nuclear gene,which was mapped to a 398 kb region between the marker Indel 4and Indel 10 on the long arm of chromosome 1using the F2 mapping population generated from the cross between mutant bf370 and Nipponbare.Sequencing analysis showed a 66 nucleotide deletion in the second exon of D10,resulting in a 22 amino acids deletion in the predicted RPE65 domain of D10 protein in the bf370 mutant.Combined with the phenotype of other d10 mutants,the phenotype of bf370 was likely caused by a 66 nucleotide deletion in D10.