通痹胶囊对膝骨关节炎患者关节功能及关节滑液基质金属蛋白酶-3、-9水平的影响

Effects of Tongbi capsule on knee joint function and synovial fluid levels of matrix metalloproteinase-3,-9 in patients with knee osteoarthritis

目的：探讨通痹胶囊对膝骨关节炎患者关节功能及关节滑液基质金属蛋白酶（matrix metalloproteinase, MMP）-3与MMP-9水平的影响。方法将76例膝骨关节炎患者按随机数字表法分为2组各38例。对照组口服盐酸氨基葡萄糖胶囊，观察组在对照组基础上加服通痹胶囊。2组均治疗12周。采用西安大略和麦克马斯特大学骨关节炎指数评价关节功能，酶联免疫吸附测定法检测关节滑液中MMP-3、MMP-9水平。治疗前后分别检测关节滑液中MMP-3、MMP-9水平，评价关节功能。结果观察组总有效率为94.74%（36/38），对照组为78.95%（30/38），2组比较差异有统计学意义（χ^2＝4.146，P＜0.05）。治疗后，观察组关节滑液中 MMP-3[（67.58±22.35）ng/ml 比（93.51±27.84）ng/ml，t＝4.477]和MMP-9[（36.24±10.56）ng/ml 比（51.87±12.35）ng/ml，t＝5.930]水平均较对照组显著降低（P 均＜0.01）。结论通痹胶囊可改善膝骨关节炎患者关节功能，其机制可能与降低关节滑液MMP-3、MMP-9水平有关。

Objective To investigate the effects ofTongbicapsule on the knee joint function and the synovial fluid levels of matrix metalloproteinase （MMP）-3,-9 in patients with knee osteoarthritis.Methods A total of 76 patients with knee osteoarthritis were randomly divided into a control group and an observation group according to the random number table, 38 in each group. Patients in the control group were treated with glucosamine hydrochloride capsules, while patients in the observation group were givenTongbicapsule on the basis of the control group. Both groups were treated for 12 weeks. The Western Ontario and McMaster Universities Osteoarthritis Index was used to measure the knee joint function, and enzyme-linked immunosorbent assay was used to determine the synovial fluid levels of MMP-3 and MMP-9.Results The knee joint function in the observation group was significantly improved than that in the control group （94.74%vs. 78.95%;χ^2=4.146, P〈0.05）. The synovial fluid levels of MMP-3 （67.58 ± 22.35 ng/mlvs.93.51 ± 27.84 ng/ml;t=4.477,P〈0.01） and MMP-9 （36.24 ± 10.56 ng/mlvs.51.87 ± 12.35 ng/ml;t=5.930,P〈0.01） in the observation group patients were significant lower than those in the control group after treatment.ConclusionsTongbicapsule was effective in treating knee joint function in patients with knee osteoarthritis, and its mechanism may be related to its reducing the synovial fluid levels of MMP-3 and MMP-9.