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血清白细胞介素6和白细胞介素8水平及其基因多态性与非小细胞肺癌的关系 被引量：5

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摘要 目的探讨白细胞介素6（IL-6）-634位点及白细胞介素8（IL-8）-251位点的单核苷酸多态性（SNPs）及血清IL-6、IL-8水平与非小细胞肺癌（NSCLC）发病风险的关系；分析IL水平与其编码基因多态性的关系。方法选取2010年1月至2014年6月132例NSCLC患者（NSCLC组）作为研究对象，同期150名健康体检者作为对照组。采用荧光定量PCR为基础的高分辨率熔解曲线分析（HRM）技术检测IL-6-634C／G及IL-8-251T／A基因单核苷酸多态性，随机选取10％进行基因测序验证，应用ELISA法测定两组血清中IL-6及IL-8浓度。结果（1）NSCLC组血清IL．6（9．56±5．52）ng／L、IL-8（4．25±2．12）ng／L水平显著高于对照组[（2．65±2．12）、（1．12±0．83）ng／L]，差异均有统计学意义（P均〈0．05）。（2）携带IL-6-634位点CG和CG＋GG基因型人群NSCLC发病风险分别是CC型的1．760倍和1．846倍，差异有统计学意义（OR=1．760，95％CI：1．064—2．911，P=0．027；OR=1．846，95％CI：1．149—2．967，P：0．011）；IL-8—251位点TA及AA基因者的NSCLC发病风险较TT型患者有所减低，但差异元统计学意义（P〉0．05）；鳞癌与腺癌患者比较，IL-6—634C／G及IL-8．251T／A基因型分布及等位基因频率差异无统计学意义（P〉0．05）。（3）NSCLC组中IL-6—634C／G及IL-8—251T／A各基因型患者的血清IL-6及IL-8水平差异无统计学意义（P均〉0．05）。结论NSCLC患者血清IL-6及IL-8水平明显升高；IL-6．634位点携带G等位基因的个体NSCLC的发病风险可能增加；IL-6及IL-8的表达水平与IL-6．634及IL-8-251的基因多态性无明显关系。
作者 李君蕊 肖颖 付盈菊 赵宝春 张雅丽 李广平 卢玉娟
机构地区 河北医科大学附属唐山市工人医院感染内科 河北医科大学附属唐山市工人医院肿瘤内科 河北医科大学附属唐山市工人医院急诊内科 河北医科大学附属唐山市工人医院检验科
出处 《中国综合临床》 2015年第7期581-584,共4页 Clinical Medicine of China
基金 唐山市科技计划项目（14130247a）
关键词 白细胞介素6 白细胞介素8 基因多态性 非小细胞肺癌 细胞因子
分类号 R503 [医药卫生—内科学]
  • 相关文献

参考文献18

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共引文献6

同被引文献47

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引证文献5

二级引证文献7

中国综合临床
2015年 第7期

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