Wnt3a联合BMP7对BMSC成骨分化的初步研究

A Preliminary Investigation into the Differentiation Effect of Wnt3a in Combination with BMP7 on BMSC Osteogenesis

目的:探讨Wnt蛋白(Wnt3a)联合骨形态发生蛋白(BMP7)对骨髓间充质干细胞(BMSCs)成骨分化的作用。方法:选择SD大鼠12只,进行骨髓间充质干细胞的分离、纯化、培养,并采用不同的方法进行骨髓间充质干细胞的体外诱导分化,按干预的不同分为4组:Wnt3a组、BMP-7组、联合诱导组以及对照组,对比不同方法对成骨分化的诱导作用差异。结果:联合诱导组的IA值为(121627±261),显著低于Wnt3a组、BMP-7组、对照组的(147173±348)、(146557±361)、(182659±409),并且其有大量橘红色矿化结节形成,较其他三组更多,组间差异比较均具有统计学意义(P<0.05)。结论:Wnt3a联合BMP7诱导BMSC成骨分化的效果显著优于单独采用Wnt3a或BMP7进行诱导,值得在临床上推广使用。

Objective： To Investigate the differentiation effect of Wnt3a in combination with BMP7 on BMSC osteogenesis. Methods：Select 12 SD rats ,detach ,purify and cultivate BMSCs,externally induct and differentiate BMSCs with different methods, divide them into 4 groups according to the interventions： Wnt3a group,BMP-7 group,associated group and control group ,compare the differences of different methods in in- ducting the differentiation of osteogenesis. Results：The IA value of the associated group was obviously higher than that of the Wnt3a group,BMP-7 group and control group, the figure was （121627!261）,（147173~ 348） ,（146557＋361）,（182659~409）respectively. And a large number of orange mineralized nodules formed in associated group, which was much more than that in the other groups , to compare these groups, the differ- ences were statistically significant（P〈0.05）. Conclusion：It is more effective to induct BMSC osteogenesis to differentiate with Wnt3a in combination with BMP7 than with Wnt3a or BMP7 alone,it is worthy of clinical promotion and application.