人参皂苷Rd治疗大鼠溃疡性结肠炎给药途径的研究

Study on the administration route of ginsenoside Rd on ulcerative colitis in rats

目的:研究人参皂苷Rd(Rd)治疗大鼠溃疡性结肠炎(UC)的最佳给药途径。方法:用炎症诱导剂三硝基苯磺酸(TNBS)溶液诱导法建立大鼠溃疡性结肠炎模型。将Wistar雄性大鼠随机分为模型对照组、Rd灌胃给药组、Rd肌肉注射组。Rd给药剂量为30mg/kg,每天给药1次,连续给药7天。结果:1与模型对照组比,Rd灌胃给药组及Rd肌肉注射组大鼠结肠湿重指数均明显减轻,差异具有统计学意义(P<0.01或P<0.05),其中灌胃给药组大鼠结肠湿重指数最低。2与模型对照组比,Rd灌胃给药组及Rd肌肉注射组能够明显降低UC大鼠结肠大体观察评分及病理组织学评分,减轻大鼠结肠的病理学损伤,差异具有统计学意义(P<0.01)。结论:人参皂苷Rd对大鼠溃疡性结肠炎有明显的治疗作用,且灌胃给药作用更强。

Objective： The aim of the study was to investigate the therapeutic effect of administration route of ginsenoside Rd in the treatment of ulcerative colitis（UC）. Methods：Colitis in rats was induced by colonic administration with 2,4,6-trinitrobenzene sulfonic acid（TNBS）.Wistar rats were randomly divided into three groups：TNBS control group, ginsenoside Rd intragastric group（ig at 30mg/kg ginsenoside Rd）and intramuscular injection group（im at 30mg/kg ginsenoside Rd）. Rats were administrated by ginsenoside Rd（30mg/kg）per day for 7days with different administration route. Macroscopic and microscopic damage scores and changes in weight/length ratio（mg/mm）of colon segments were analyzed. Results：1.Compared with the TNBS control group, Rd ig group and im group facilitated recovery of pathologic changes in the colon and decreased colonic weight/length ratio（P 0.01 or P 0.05）. 2.Rd also notably reduced the colonic macroscopic and microscopic damage scores（P0.01）. Conclusion：Rd exerts a beneficial effect in the TNBS-induced colitis in rats and compared with intramuscular injection, the administration of Rd by ig has a better effect on ulcerative colitis in rats.