摘要
【目的】探讨表皮生长因子受体(ErbB4)在糖尿病大鼠海马区星形胶质细胞中的表达及意义。【方法】SD 大鼠单次腹腔注射链脲佐菌素(STZ)诱发实验性1型糖尿病,检测 ErbB4和胶质纤维酸性蛋白(GFAP)在海马区星形胶质细胞中的表达;高糖孵育星形胶质细胞,观察 ErbB4在细胞中的变化。【结果】与对照组相比,实验性1型糖尿病大鼠海马区 GFAP 阳性的星形胶质细胞数目明显增加( P <0.05),ErbB4阳性的星形胶质细胞数目明显减少( P <0.05),ErbB4/GFAP 双标阳性细胞明显低于对照组( P <0.05)。在培养的原代星形胶质细胞,高糖增加细胞活性(P <0.05),且明显地降低 ErbB4蛋白表达( P <0.05),但不改变其分布。【结论】糖尿病海马区星形胶质细胞 ErbB4蛋白的表达明显降低,高糖可能是诱导糖尿病海马区星形胶质细胞 ErbB4蛋白表达减少的原因。
[Objective] To determine the expression and significance of ErbB4 in hippocampal astrocytes in streptozotocin‐induced diabetic rats .[Methods] Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin in Sprague Dawley rats .Serum concentrations of glucose were measured and ErbB 4 im‐munoreactivity was detected in hippocampal astrocytes .In cultured cortical astrocytes ,immunohistochemistry was performed to examine the morphological alteration under a stimulation of high glucose (HG) and monitor the changes in ErbB4 expression .[Results] ErbB4 expression was reduced in hippocampal astrocytes of dia‐betic brains .And HG treatment induced morphological changes of cultured astrocytes and caused a down‐regu‐lation of ErbB4 .[Conclusion] ErbB4 immunoreactivity significantly decreases in hippocampal astrocytes of diabetic rats .In cultured astrocytes ,HG may down‐regulate the expression of ErbB4 .
出处
《医学临床研究》
CAS
2015年第6期1063-1066,共4页
Journal of Clinical Research
基金
长沙市科技局项目资助(k1403062-31)