芍药苷对黑色素瘤A-375细胞凋亡的影响

Mechanism and Apoptotic Effect of Paeoniflorin in Melanoma Cell A-375

【目的】探讨芍药苷对黑色素瘤 A‐375细胞凋亡的影响。【方法】采用不同浓度（0．5、1、2 mg／mL）芍药苷处理黑色素瘤 A‐375细胞后继续培养24 h ，MTT 法检测细胞抑制率，Hoechst 染色检测细胞形态， Western Blot 检测抑癌基因 Bax 、细胞增殖抗原（PCNA）、核因子κB（NF‐κB） p65蛋白表达。【结果】随着给药浓度的增加，芍药苷对 A‐375细胞的抑制率逐渐增高，在48 h 达到高峰；Hoechst 染色结果表明随着浓度的逐渐增高，细胞裂解强度增大；Western Blot 结果表明芍药苷上调 Bax 表达，下调 PCNA 表达，抑制 NF‐κB p65表达，且呈剂量依赖性。【结论】芍药苷诱导黑色素瘤 A‐375细胞凋亡，可能是通过 NF‐κB 信号通路发挥作用。

[Objective] To explore the apoptotic effects and mechanism of paeoniflorin in melanoma cell A‐375 .[Methods] Melanoma cell A‐375 was treated with paeoniflorin （0 .5 ,1 ,2 mg/mL） .The cell apoptotic rate was detected by methyl thiazolyl tetrazolium （MTT ） assay .Cellular morphology was examined by Ho‐echst staining .The expressions of Bax ,PCNA and NFκB p65 were assessed by Western blot .[Results] M TT assay demonstrated paeoniflorin （0 .5 ,1 ,2 mg /mL） could reduced cell viability .And inhibitory effect peaked at 48h .Hoechst staining indicated that paeoniflorin could induce cell apoptosis in a dose‐dependent way .And paeoniflorin could down‐regulate the expressions of PCNA and NFκB p65 and up‐regulate the expression of Bax in a concentration‐dependent way .[Conclusion] Paeoniflorin induces apoptosis through a NF‐κB signal pathway in melanoma cell A‐375 .