水稻抗褐飞虱种质Swarnalata抽穗期和休眠性相关QTL检测

Detection of Quantitative Trait Loci for Heading Date and Seed Dormancy in the Brown Planthopper Resistance Cultivar Swarnalata(Oryza sativa L. )

为有效利用抗褐飞虱水稻Swarnalata,对2013年南京种植的Swarnalata/02428 F2分离群体进行抽穗期和种子休眠性考察,利用172个分子标记构建了Swarnalata/02428 F2的分子遗传连锁图谱,图谱全长为3311.4c M,标记间平均图距为19.22c M。利用Windows QTL Cartographer V2.5软件对该分离群体进行抽穗期和种子休眠性相关QTL检测,共检测到7个抽穗期相关QTL,分别位于第2、3、6、11染色体,其中位于第11染色体的q HD-11-1贡献率最高,为28.85%;检测到3个种子休眠性相关QTL,分别位于第3、6、9染色体,其中位于第9染色体的q Sd-9贡献率最高,为22.11%。分析表明,本研究检测到的抽穗期QTL与种子休眠QTL所在位置不同,说明该群体中种子休眠与抽穗期没有直接关系,它们分别由不同基因控制。本研究不仅为水稻休眠基因的精细定位及克隆奠定基础,也为更有效利用Swarnalata中的抗褐飞虱基因提供基础和一些优良的中间材料。

In the present study,one F2 population derived from brown planthopper resistance cultivar Swarnalata /02428 was planted in 2013 at Nanjing Agricultural University Rice Station（ Tuqiao,Nanjing,China）. Phenotype values of heading date and seed dormancy in the F2 population were investigated. Using 172 markers,a molecular genetic map of the F2 population was constructed,which total length was 3311. 4c M and an average marker interval distance was 19. 22 c M. QTL controlling heading date and seed dormancy was detected in this F2 population by using QTL Cartographer version 2. 5 software. A total of 7 QTL related to heading date were identified. They were located on chromosomes 2,3,6 and 11,respectively. Among them,a QTL named q HD 11-1 was flanked by markers RM286 and Indel 11-2 on chromosome 11,which explained 28. 85% of total phenotypic variation with a LOD score of2. 89. Meanwhile,3 QTL for seed dormancy were found. They were located on chromosomes 3,6 and 9,respectively.Among them,a QTL named q Sd-9 was located between marker RM219 and RM1328 on chromosome 9 with relative high LOD values of 3. 11 and explained phenotypic variations of 22. 11%. Results in this study suggest that seed dormancy and heading date are controlled by different genetic factors. These results provide an opportunity for map-based cloning major QTLs for seed dormancy and heading date or materials for pyramiding program in the brown planthopper resistance rice breeding.