基于SSR标记的六倍体小黑麦遗传多样性分析

Genetic Diversity of Hexaploid Triticale Analyzed by SSR Markers

采用已筛选出的多态性较好的分布于六倍体小黑麦21对染色体上的100对SSR引物对从国际玉米小麦改良中心（CIMMYT）引进的339份六倍体小黑麦进行了遗传多样性分析。结果显示,100对引物共检测出323个等位变异,变幅是1-6个,平均等位变异丰富度为3.23;多态信息含量（PIC）的变幅为0-0.748,平均多态信息含量为0.465;平均遗传多样性指数（H＇）为0.1439,说明供试的339份六倍体小黑麦品种的SSR遗传多样性较为丰富。同时基于Nei＇s遗传距离对339份材料进行聚类分析,可以将供试材料分为6个类群。其中,第Ⅵ类群与其他类群的遗传距离较大、遗传差异较明显。研究结果为将六倍体小黑麦的优良基因应用于普通小麦的遗传改良提供了理论依据。

Genetic diversity of 339 Hexaploid Triticale from International Maize and Wheat Improvement Center（ CIMMYT）,was analyzed based on 100 microsatellite markers distributed across the whole Triticale genome. A total of 323 alleles were detected with 3. 23 alleles per locus. The polymorphic information content（ PIC） ranged from 0to 0. 748,with an average of 0. 465 and the average genetic diversity index（ H＇） was 0. 1439. These indicated that genetic diversity of 339 tested cultivars was rich based on SSR markers. Clustering results based on Nei＇ s genetic distance showed that the tested materials could be divided into six groups,and groups Ⅵ was clearly distinct from the other species. The results would provide a theoretical basis on genetic improvement of wheat by using fine genes from Hexaploid Triticale.