γ射线辐射“9311”水稻突变体的筛选

Screening of Mutant for Rice Cultivar “9311”by γ-ray Irradiation

突变体的创建和筛选是挖掘基因功能的重要来源,也是扩充种质资源的有效途径之一。利用60Coγ射线处理籼稻"9311"种子,经过M2筛选及M3鉴定,共获得128份突变体,突变频率为10.24%。突变类型包括生育期及育性突变、叶片性状突变、茎秆形态突变、穗部形态突变、子粒形态突变等,此外,还发现一个植株变矮、节间和叶片变短且不能进入生殖生长的突变体,该突变体在湖北和海南连续种植都不能开花结实。筛选的突变体不仅为水稻功能基因组学研究提供丰富的材料,部分突变体还可直接作为育种材料应用。

Creation and screening of mutants are an important source of mining gene,and also are one of the effective ways to expand the germplasm resources. In this paper,indica rice cultivar 9311 seeds were treated with60 Co γ-ray,and 134 mutants were obtained by M2 generation screening and M3 generation identification,and the mutation frequency was 10. 24%. The types of mutation included growth period and fertility,leaf traits,stem morphology,panicle traits,grain shape,and so on. Moreover,we also found a dwarf mutant with shorter internodes and leaves and without reproductive growth,and the mutant could not blossoming and fruiting after continuously planting in Hubei and Hainan province. The mutants provided abundant materials for rice functional genomics study,and some of them could be directly used as breeding materials.