摘要
半乳糖凝聚素-3(Galectin-3,Gal-3)是一种多功能蛋白,参与机体内的多种生命活动过程。为分析大鼠Rattus norvegicus gal-3 c DNA分子多样性,在分析Gen Bank注册的大鼠gal-3 c DNA序列的同时,从三只不同大鼠个体的肝脏第一链c DNA,共计9个独立实验中克隆gal-3开放阅读框OFR(Open reading frame)。分析结果表明,在已注册的序列中存在1个单核苷酸多态性(Single nucleotide polymorphism,SNP)。本实验从1-3号大鼠肝脏分别获得118、95和120个克隆,新发现1处缺失和5处SNP,共克隆17个不同的gal-3 ORF,其中仅1个克隆与已报告大鼠gal-3 c DNA的ORF完全相同。从1号和3号个体肝脏中分别克隆11和14个不同的gal-3 ORF,揭示大鼠gal-3 c DNA存在个体水平的分子多样性。
Galectin-3（ Gal-3） is a multi-functional protein involved in many biological processes. To clarify the gal-3 cDNA diversity of Rattus norvegicus,cloning of gal-3 open reading frame（ ORF） from liver first strand cDNA of three individuals was performed totally from 9 independent experiments. Consequently,118,95 and 120 clones were obtained from three individuals,respectively. Alignment of all these sequences indicated 1 deletion and 5single nucleotide polymorphism（ SNP） sites,and 17 different clones were obtained. Nine out of the 17 clones appeared in two or more independent experiments,and 11 and 14 different clones were obtained from the first and the third individuals,respectively. These results indicated the objectivity of liver gal-3 cDNA diversity at individual level in R. norvegicus.
出处
《生物信息学》
2015年第2期88-95,共8页
Chinese Journal of Bioinformatics
基金
国家自然科学基金资助项目(31372149)
国家级大学生创新创业训练计划项目(201310341017)
