结核性胸积液腺苷脱氨酶2活性及免疫组化研究

Study on the Activity and Immunohistochemistry of Adenosine Deaminase 2 in Tuberculosis Pleural Effusion

目的:研究腺苷脱氨酶2(Adenosine deaminase 2,ADA2)在结核性胸积液总ADA活性所占比例,及其在结核性胸积液胸膜组织中的细胞来源。方法:测定确诊的32例x结核性胸积液ADA2的总ADA和ADA2活性,以ADA2抗体为一抗,用免疫组化方法检测ADA2在结核性胸积液胸膜组织中的细胞来源。结果:ADA2活性占总ADA的74.8%。结核性胸积液胸膜组织的ADA2免疫组化,可见朗格汉斯巨细胞、上皮样细胞和巨噬细胞被深染,胞浆和胞膜有大量的棕黄色沉着。结论:结核性胸积液的ADA活性主要由ADA2构成,ADA2源自巨噬细胞、上皮样细胞和朗格汉斯巨细胞。

Objective：To investigate the proportion of Adenosine deaminase 2（ADA2） in total ADA activity of the tuberculosis pleural effusion and its cellular source in pleural tissue.Method：The total ADA and ADA2 activity from thirty-two tuberculosis patients＇ effusions were measured.The cellular source of ADA2 were examined by immunohistochemistry method using CECR1 antibody.Result：The ADA2 contributed 74.8% to the total ADA.The immunohistochemistry of ADA2 in the pleural tissues of tuberculous pleural effusion showed that langhans cells,epithelioid cells and macrophages were stained deeply.Their cytoplasm and membrane were deposited by yellow granules.Conclusion：ADA2 is primarily responsible for total ADA activity in tuberculosis pleural effusion and it is secreted by macrophages,epithelioid cells and langhans cells.