双丹明目胶囊对糖尿病视网膜病变大鼠视网膜VEGF和VEGFR蛋白表达的影响

Effects of Shuangdan Mingmu Capsule on the Expression of Retinal VEGF and VEGFR Protein Activity in Rats with Diabetic Retinopathy

目的观察双丹明目胶囊对糖尿病视网膜病变(diabetic retinopathy,DR)大鼠视网膜血管内皮生长因子(vascular endothelial growth factor,VEGF)和血管内皮生长因子受体(vascular endothelial growth factor receptor,VEGFR)蛋白表达的影响。方法将40只SD大鼠随机分为正常组10只,模型组30只。采用尾静脉注射链脲佐菌素(STZ)法建立DR大鼠模型,再随机分为模型对照组、双丹明目组、阳性对照组,每组10只。各组大鼠分别连续给药8周后用蛋白质印迹法(Western Blot)检测视网膜组织VEGF和VEGFR表达。结果 (1)与正常组相比,模型对照组VEGF和VEGFR蛋白表达量升高(P<0.01);(2)与模型对照组相比,双丹明目组和阳性对照组VEGF和VEGFR蛋白表达量降低(P<0.01);(3)双丹明目组和阳性对照组相比,VEGF和VEGFR蛋白表达量差异无统计学意义(P>0.05)。结论双丹明目胶囊能够降低糖尿病视网膜病变大鼠视网膜VEGF蛋白和VEGFR蛋白的表达,从而可能抑制视网膜血管新生。

Objective To observe the effects of Shuangdan Mingmu Capsule on the expression of retinal VEGF and VEGFR protein activity in rats with diabetic retinopathy（DR）. Methods 40 SD rats were randomly divided into the normal group and model group, 10 rats and 30 rats, respectively. The DR model rats were induced by intravenous injection of STZ. Then the model rats were randomly divided into the model control group, Shuangdan Mingmu group, and positive control group. The effects of Shuangdan Mingmu Capsule on the expression of rat retinal VEGF and VEGFR protein with diabetic retinopathy was tested by western blot after 8 weeks of medication. Results（1） Compared with the normal group,the expression of VEGF and VEGFR protein in model control group was increased（P〈0.01）;（2） Compared with the model control group, the expression of VEGF and VEGFR protein in Shuangdan Mingmu group and positive control group was decreased（P〈0.01）;（3） Compared with Shuangdan Mingmu group and positive control group, the expression of VEGF and VEGFR protein have no statistically significant（P〉 0.05）. Conclusion Shuangdan Mingmu capsule can down-regulate the expression of retinal VEGF and VEGFR protein in rats with DR, then the capsule may inhibit retinal neovascularization.