2008—2013年北京市17株伤寒和副伤寒沙门菌耐药和分子分型研究

Drug resistance and molecular typing for 17 strains of salmonella typhi and salmonella paratyphoid in Beijing from 2008-2013

目的分析2008—2013年北京市17株伤寒、副伤寒沙门菌耐药及分子分型。方法对2008-2013年通过对北京市肠道门诊监测系统分离到的17株伤寒、副伤寒沙门菌进行生化鉴定、血清分型并运用纸片法进行药敏检测;利用脉冲场凝胶电泳(PFGE)进行分子分型。结果 17株伤寒、副伤寒沙门菌对三代头孢菌素类药物头孢他啶敏感率达100%,头孢噻肟和头孢曲松敏感率均为93.75%,对氟喹诺酮类药物环丙沙星敏感率为93.75%,萘啶酸耐药率达31.25%。其中16株伤寒、副伤寒沙门菌可分为15种PFGE带型,带型分布较分散。结论北京市伤寒、副伤寒沙门菌分离株对3代头孢类抗生素及环丙沙星敏感;PFGE分子型别较多,无聚集性。

[Objective] To study the drug resistance and molecular typing for 17 strains of salmonella typhi and salmonella paratyphoid in Beijing from 2008-2013.[Methods]17 strains of salmonella typhi and salmonella paratyphoid, which were isolated in gastrointestinal clinic surveillance system in Beijing from 2008-2013, were identified by biochemical tests and serological typing. The drug resistance was detected by disk diffusion method and molecular typing was performed by pulsed-field gel electrophoresis（PFGE）.[Results] The susceptibility rates of 17 strains of salmonella typhi and salmonella paratyphoid to ceftazidime reached 100%, susceptibility rates to cefotaxime and ceftriaxone were both 93.75%, and the susceptibility rate to ciprofloxacin（fluoroquinolones） was 93.75%, while resistant rate to nalidixic acid was 31.25%.16 strains of salmonella typhi and salmonella paratyphoid were divided into 15 PFGE band types, and the belt type distribution was scattered.[Conclusion]Strains of salmonella typhi and salmonella paratyphoid in Beijing are susceptible to third-generation cephalosporins and ciprofloxacin, and there is no aggregation in PFGE molecular typing.