乙型肝炎前S1、S2抗原与HBV-DNA的相关性分析

Relationship between hepatitis B virus former S1 antigen,former S2 antigen,and HBVDNA,and their clinical significance

目的对比分析乙型肝炎(乙肝)前S1、S2抗原和HBV-DNA的相关性,探讨乙肝前S1、S2抗原的临床应用价值。方法随机选取乙肝患者420例,采用ELISA法对前S1、前S2抗原进行检测,采用荧光定量PCR对HBV-DNA进行检测,从血清学标志类型、HBV-DNA载量和HBV感染类型角度对HBV-DNA和前S1、S2抗原检测结果进行分析。结果乙肝大三阳患者乙肝前S1、S2抗原和HBV-DNA阳性率分别为98.48%、95.45%、100.0%;小三阳患者乙肝前S1、S2抗原和HBVDNA阳性率分别为53.25%、45.45%、46.75%。HBs Ag(+)HBc Ab(+)患者乙肝前S1、S2抗原和HBV-DNA阳性率分别为53.75%、43.28%、44.78%。在高HBV-DNA载量组中乙肝前S1、S2抗原检出率较高。结论乙肝前S1、S2抗原与HBV-DNA有较好的相关性,可作为反映病毒感染与复制的指标。

Objective To study the relationship between HBV Pre-S1 antigen,HBV Pre-S2 antigen and HBV-DNA and inves-tigate the clinical significance of the hepatitis B virus （ HBV） .Methods The markers Pre-S1 antigen, Pre-S2 antigen were deter-mined by ELISA and HBV-DNA by the fluorescence quantitative PCR in 420 patients with HBV collected from September 2013 to May 2014.HBV, Pre-S1 antigen and Pre-S2 antigen results were analysed in different aspects, such as serum indices, HBV-DNA amounts and HBV-DNA infection types.Results In “big sanyang” patients the positive rates of Pre-S1, S2 antigen and HBV-DNA were 98.48%, 95.45%, 100%,respectively.In“small Sanyang” patients, the positive rates of Pre-S1, S2 antigen and HBV-DNA were 53.25%, 45.45%, 46.75%,respectively.In HBsAg＋HBcAb＋patients the positive rate of Pre-S1, S2 antigen and HBV-DNA were 53.75%, 43.28%, 44.78%,respectively.And the detection rates of HBV Pre-S1 antigen, HBV Pre-S2 antigen were higher in pa-tients infected with high level of HBV-DNA.Conclusions There is a good correlation between HBV Pre-S1 antigen, HBV Pre-S2 an-tigen and HBV-DNA, which can reflect virus infection and replication.