高效液相色谱法测定UNASYN的血药浓度

HIGH PERFORIVIANCE LIQUID CHROHATOGRAPHIC ASSAY OF UNASYN IN SERUM

建立了反相离子对高效液相色谱法测定Unasyn中氨苄青霉素血药浓度。样品经酸法转入有机相,在中性条件下转入水相。色谱柱ODS-C18反相柱,流动相:甲醇/水(36/65)含0.01mol/L四甲基氯化铵,pH3.5。采用UV检测器,(λ=206nm)得出检测下限0.02μg,最低检出血药浓度1.0μg/ml,线性范围3.125-50μg/ml,抽提效率82.55％。本方法简便、灵敏、专一、适合用于Unasyn血药浓度测定。

We report a reversed, ion-pair high performance liquid chromato- graphy for the assay of Unasyn in serum. The acid-treated sample was first extracted into organic phase and then re-extracted into aqueous phase at pH 7.0. Chromat-ographic column was ODS-C18. Mobile phase con sisted of 0.01 M tetfamethylammo-nium chloride and 0.01 M tribasic sodium phosphate in methanol/water(35/65, v/v), pH 3.5. The column eluent was monitored at 206nm. Detection limit 'was 0.02/ue. The method was linear from 3.125-50 μg/ml (r=0.9984). The extraction efficiency was 82.55%. This method was simple and sensitive, and could be applied to the clinical detection of Unasyn in serum.