胡杨和灰叶胡杨不同器官寄藏的可培养微生物种类研究

Study on the Culturable Microbes from Different Organs of Populus euphratica and P. pruinosa

采用洗涤法和平皿检测等方法,研究了胡杨和灰叶胡杨不同器官寄藏的可培养微生物种类。结果显示,胡杨种子内部和外部带菌量显著高于灰叶胡杨;灰叶胡杨的芽、叶柄及枝条带菌量均高于胡杨,其中叶柄、枝条上部和中部带菌量显著高于胡杨;胡杨和灰叶胡杨枝条带菌率由低到高依次为:上部<中部<下部,且两个物种枝条下部带菌率均达100%。从胡杨及灰叶胡杨种子、芽、叶柄及枝条各器官分离到可培养的真菌7株、细菌2株;经形态学及分子生物学方法鉴定,分别为Alternaripp.(链格孢属,3种)、Aspergillus spp.(曲霉属,2种)、Nigrospora sp.(黑孢霉属)、Epicoccum sp.(附球菌属)、Stenotrophomonas spp.(嗜麦芽窄食单胞菌属,2种),其中黑孢霉属和附球菌属真菌系胡杨和灰叶胡杨内生菌首次报道;链格孢属、曲霉属真菌及嗜麦芽窄食单胞菌属细菌为胡杨和灰叶胡杨种子外部携带可培养微生物首次报道。

The culturable microbes from different organs of Populus euphratica and P. pruinosa were studied by using washing and plating tests. The results showed that the quantity of microbes associated with seeds of P. euphratica was significantly higher than that of P. pruinosa. Compared with P. pruinosa,the rates of fungi associated with the organisms of buds,petioles,and branches of P.euphratica were lower,and significant higher in that of petioles,upper and central branches. For the both kinds of tree species,the rates of fungi associated with lower branches were up to 100 %,followed by that of central and upper branches. Seven fungal strains and two bacterial strains were isolated from seeds,buds,petioles and branches of both P. euphratica and P. pruinos. Based on the methods of morphology and molecular biology,Alternaria spp.（3 strains）,Aspergillus spp.（2 strains）,Nigrospora sp.,Epicoccum sp. and Stenotrophomonas spp.（2 strains） were determined. It was first reported that Nigrospora and Epicoccum were endophytic fungi,Alternaria,Aspergillus and Stenotrophomonas were seed associated fungi and bacteria of P. euphratica and P. pruinosa.