摘要
本文研究建立了鱿鱼丝中氯霉素含量的液相色谱串联质谱测定方法。样品经过加入同位素内标后,用甲醇直接提取后,注入液相色谱串联质谱仪中进行检测。利用ESI源（负离子模式）,C18液相色谱柱,以及SRM方法对样品中的氯霉素进行定性,定量分析。该方法测定结果的相对标准偏差小于6%（n=6）,平均回收率大于90%,线性范围为1-10ng/m L,最低检出限为0.031ng/m L。
A method for determination of Chloramphenicol residue in Shredded squid by isotope internal standard-liquid chromatography-Tandem mass spectrometry（LC-MS/MS）was established.The sample was injected into LC-MS/MS after extracted by methanol.The quantitative analysis was achived by ESI source under negative ion mode,C18 liquid chromatography column.The RSD of the method was less than 6%,and average recovery was more than 90%.the liner range was in 1-10ng/m L and the low detection limit was 0.031 ng/m L.
出处
《生命科学仪器》
2015年第3期42-45,共4页
Life Science Instruments
