手足口病患儿血清循环脱氧核糖核酸和 C 反应蛋白水平的变化及意义

Alteration and clinical significance of cfDNA and CRP in children patients with hand -foot -and -muoth disease

目的：探讨血清循环脱氧核糖核酸（cfDNA）和 CRP 水平在手足口病（HFMD）患儿中的变化及其临床意义。方法采用荧光定量 PCR（FQ-RT-PCR）和免疫比浊法测检108例手足口病患儿血清 cfDNA 和CRP 浓度，并以108例健康人作为对照。结果HFMD 组血清 cfDNA、CRP 水平分别为（523．45±42．31）mg／L、（22．57±5．52）mg／L，对照组血清 cfDNA、CRP 水平分别为（260．18±20．56）mg／L、（2．88±0．82）mg／L，两组差异均有统计学意义（t ＝58．16、36．85，均 P ＜0．05）；血清 cfDNA 和 CRP 水平随着临床表现逐步加重逐渐升高（t ＝29．80、13．68，均 P ＜0．05）；血清 cfDNA 水平与血清 CRP 水平水平呈正相关（r ＝0．730，P ＜0．01）。结论血清 cfDNA 及 CRP 水平反映了手足口病病情轻重程度，与手足口病的临床变化有较密切的关系，对病情和预后判断有重要价值。

Objective To explore the clinical significance of changes of serum cfDNA and CRP in patients with hand -foot -and -muoth disease.Methods Serum levels of cfDNA,CPP were measured with fluorescent quan-titation PCR and immunoturbidime,respectively in 108 cases of children patients with hand -foot -and -muoth and 108 healthy subjects children as control group.Results Serum cfDNA and CPP levels with hand -foot -and -muoth group（523.45 ±42.31）mg/L,（22.57 ±5.52）mg/L were significantly higher than those of control group detection results（260.18 ±20.56）mg/L,（2.88 ±0.82）mg/L（t =58.16,36.85,all P 〈0.05）.And we observed the serum cfDNA and CPP levels in different severity extent of pneumonia,the results show that the serum increased gradually with the increase of the severity（t =29.80,13.68,all P 〈0.05）.and the serum level of cfDNA was positive correla-tion with CRP（r =0.730,P 〈0.01 ）.Conclusion The serum.cfDNA and CRP reflect situation of patients and extent of hand -foot -and -muoth disease,which have a close relationship between the clinical process of hand -foot-and -muoth disease and have significance value to evaluate the pathogenetic condition and prognosis.