布鲁杆菌病患者T淋巴细胞亚群的分析

Analysis of lymphocyte T subgroup in Brucella patients

目的探讨布鲁杆菌病(布病)患者T淋巴细胞亚群的变化,为判断布病患者免疫功能变化提供依据。方法 142例布病患者及45例健康体检人群(对照组)血样标本来自于黑龙江省农垦总局总医院检验科。采用流式细胞术方法测定CD3+淋巴细胞百分比、CD19+淋巴细胞百分比、CD3+/CD4+T淋巴细胞百分比、CD3+/CD8+T淋巴细胞百分比、CD4+/CD8+比值,同时进行统计学分析。结果与对照组比较布病组CD3+%增多、CD19+%减低、CD3+/CD4+%减低,CD3+/CD8+%增多,CD4+/CD8+比值减低,且P<0.05变化有显著性意义。结论布病患者细胞免疫及体液免疫功能均发生变化。

Objective To investigate the variation of lymphocyte subgroup in patients with Brucellosis , Provide the basis for judging the changes of immune function in patients with Brucellosis .Methods All the blood samples came from clinical laboratory of Heilongjiang Agricultural Reclamation Bureau General Hospital . Including 142 Brucella patients and 45 healthy people （ control group ） .Flow cytometric method was used to determine the percentage of CD3 ＋lymphocyte, the percentage of CD19 ＋lymphocyte, the percentage of CD3 ＋/CD4 ＋lymphocyte, the percentage of CD3 ＋/CD8 ＋lymphocyte and CD4 ＋/CD8 ＋ratio.Statistical analysis was carried on at the same time .Results CD3 ＋cells percentage in the Brucella group was increased compared with the control group , CD19 ＋, cells percentage in the Brucella group was decreased compared with the control group, CD3 ＋/CD4＋ cells percentage in the Brucella group was decreased compared with the control group , CD3 ＋/CD8 ＋cells percentage in the Brucella group was increased compared with the control group , CD4 ＋/CD8 ＋ratio in the Brucella group was decreased compared with the control group ,and P〈0.05 Significant changes. Conclusions Cellular immunity and humeral immunity functions are changed in different on the patients with Brucellosis .