摘要
目的探讨全自动DNA-ICM对腹盆腔冲洗液细胞学的辅助诊断作用。方法连续收集腹盆腔术中冲洗液细胞学光镜诊断后剩余标本进行DNA-ICM检测,以术后病理结合光镜下细胞形态诊断作为金标准,评价DNA-ICM的临床应用价值。结果 83例标本中72例DNA-ICM制片扫描结果满意纳入研究。术后证实恶性冲洗液16例,其中细胞学阳性12例,DNA-ICM阳性5例,细胞学和DNA-ICM共同诊断3例,2例为细胞学阴性但DNA-ICM阳性。在病理未证实恶性的冲洗液中,DNA-ICM检测阳性4例,其中2例术后病理证实有恶性肿瘤但无腹腔转移,1例有癌手术史,但本次术后病理未见癌,1例为交界性肿瘤。细胞学和DNA-ICM检测的敏感性分别为75%(12/16)和31.3%(5/16)。术后病理良性肿瘤或病变中细胞学和DNA倍体分析均未见阳性。结论冲洗液中DNA-ICM检测的敏感性不高,不能取代细胞学,但对细胞学诊断有一定的辅助作用。
Objective To explore the auxiliary diagnostic role of the automatic DNA-ICM for abdominal and pelvic lavage cytology. Method The remaining samples of liquid-based cytology after diagnosis by cytology were collected consecutively and were detected by DNA-ICM. The histopathologic and cell morphology results were acted as a gold standard to assess the value of clinical application of DNA-ICM. Result Of 83 cases of specimens,72 cases showed satisfactory DNA-ICM scan results and were included in the study. The pathologic results displayed that 16 cases were diagnosed as malignant tumor. Of these 16 cases,12 were diagnosed as malignant tumor by the cytology and only 5 were malignant by DNA-ICM. In 5 malignant cases diagnosed by the DNA-ICM,3 cases also were diagnosed malignant and the other 2 cases were negative by cytology. There were four cases were classified as malignant tumor by DNA-ICM,but had no malignant pathologic evidence. They included two malignant tumors without peritoneal metastases and one borderline tumor; another case had a history of carcinoma and had no tumor in this time of operation. The sensitivity of cytology and DNA-ICM were 75%（ 12 /16） and 31. 3%（ 5 /16）. For benign tumor or lesions confirmed by histopathology,no abnormality was found by cytology and DNA-ICM. Conclusion The sensitivity of DNA-ICM has not high enough in the diagnostic of lavage,it cannot Substitute cytology diagnosis,But it has a certain auxiliary diagnostic value for lavage cytology.
出处
《中国临床医生杂志》
2015年第6期25-28,共4页
Chinese Journal For Clinicians
基金
中国癌症基金会北京希望马拉松基金课题(LC2011B22)
