护肝片中5种木脂素成分的高效液相-荧光检测研究

Simultaneous Determination of Five Lignin of Hugan Tablets by HPLC and Fluorescence Detection

目的：建立同时测定护肝片中五味子醇甲、五味子醇乙、五味子甲素、五味子乙素和五味子丙素含量的HPLC－FLD方法。方法：用Cosmosil C18（4．6mm ×250mm，5μm）色谱柱，甲醇（A）－水（B）（85：15）等度洗脱；流速：0．6mL／min；柱温：35℃；进样量5μL，检测波长：254nm和330nm分别为激发波长和发射波长。结果：5个活性成分分离度良好，各成分质量浓度与峰面积在测定范围内均呈良好的线性关系（R2≥0．9990）；加样回收率在84．21％～105．04％范围内；RSD值在1．17％～2．18％范围内。结论：本研究建立的方法简单可靠，具有良好的精密度、重现性和稳定性，可用于护肝片中主要活性成分的同时定量分析，为不同产地、不同批次的护肝片的质量评价和质量控制提供了科学有效的依据。

Objective：To establish a simultaneous determination of five markers, namely schisandrol A and B, schisan-drin A-C for evaluation and quality control of Hugan tablets by high performance liquid chromatography （HPLC） com-bined with fluorescence detection （ FLD） .Methods：The simultaneous HPLC-FLD analysis of Hugan tablets was recor-ded at excitation wavelength of 254nm and emission wavelength of 330nm by using a reversed phase C18 column （4.6mm ×250mm, 5 μm） by an isocratic elution mobile phase consisting of methanol and water （85：15） within 25min at 35℃with a flow rate of 0.6mL/min.Results： In the quantitative analysis, all calibration curves of five selected markers showed good linear regression （ R2≥0.999 0） within the tested ranges, and the recovery of the method was in the range of 84.21%~105.04%, with RSD values ranging from 1.17%to 2.18%.Conclusion：The results demonstrate that the developed method is a rapid and effective analytical method for the quality assessment of Hugan tablets.