丹葛保肝复方提取工艺的研究

Extraction Process of Salvia and Pueraria Hepatoprotective Compound

目的:筛选最佳的丹葛保肝复方的提取工艺。方法:通过正交试验,利用高效液相色谱法同时测定不同的提取工艺后丹葛保肝复方提取物中丹酚酸B和葛根素的含量,以丹酚酸B和葛根素含量、干浸膏得率为考察指标,考察溶剂用量、提取次数、提取时间对提取效果的影响,优选最佳提取工艺。结果:优选出的最佳工艺为25倍量蒸馏水,浸泡60min,回流提取2次,每次2.5h。结论:优选的提取工艺对各指标性成分的提取率均较高,可以为制剂生产提供依据。

Objective：To sort out the best method for the extraction process of Salvia and Pueraria hepatoprotective com-pound.Methods：After orthogonal experiment, use HPLC to simultaneously determine the contents of salvianolic acid B and Puerarin, with the contents of salvianolic acid B and Puerarin, dry extract yields as indexes, the effects of solvent a-mount, extracting times and extracting time on extraction were investigated for the optimum extraction process.Results：The optimum extraction parameters were as follows：25 times of distilled water, soaking 60 min, reflux extraction 2 times and 2.5 h each time.Conclusion：The optimized extraction process for each index component extraction rate is higher and can provide the basis for the preparation of production.