神经干细胞对AD大鼠行为及突触素Ⅰ表达的影响

The effects of neural stem cells on ethology and expression of Synapsin Ⅰ in aged rats

目的:探究神经干细胞(NSCs)移植对β-淀粉样蛋白(Aβ1-42)诱导的阿尔茨海默病(AD)大鼠学习记忆能力及海马组织内突触素I表达的影响。方法:采用β-淀粉样蛋白(Aβ1-42)注射到大鼠海马组织内建立阿尔茨海默病(AD)大鼠动物模型,通过Y迷宫测试大鼠学习记忆能力和Western blot技术检测大鼠海马组织内Synapsin I表达。结果:与对照组比较,AD模型组、细胞移植组大鼠学习记忆和大鼠海马组织内Synapsin I蛋白表达量均低(P<0.05),与AD模型组比较,细胞移植组大鼠学习记忆能力和大鼠海马组织内Synapsin I蛋白表达量均较高(P<0.05)。结论:NSCs能显著改善AD大鼠的学习记忆能力,可能与上调突触素Ⅰ的表达有关。

Objective： To explore the neural stem cells（NSCs） transplantation on Aβ1-42 induced effects of learning and memory ability and hippocampus Synaptophysin I expression in alzheimer＇s disease rat.Methods： A β1-42 injection method was used to establish rat hippocampus in Alzheimer＇s disease（AD） rat animal model.Rat＇learning memory ability was tested by Y maze mehod.The I expression in hippocampus tissue of rats was detected by the technology of blot Western.Results： In AD model group,cell transplantation study group,rats＇ memory ability and the protein expression of Synapsin I in hippocampus were low compared with the control group（P〈0.05）.In cell transplantation study group,rats＇ memory ability and the protein expression of Synapsin I in hippocampus were high compared with the AD model group（P〈0.05）.Conclusion： NSCs could significantly improve the learning and memory ability of AD rats,which may be related to the upregulation of the expression of Synapsin I.